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    Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity

    Mayara Brito de Sousa2, José Otávio Carrera Silva Júnior2,3, Wagner Luiz Ramos Barbosa2, Erika da Silva Valério2, Andriele da Mata Lima4, Marlon Heggdorne de Araújo5, Michelle Frazão Muzitano5, Celso Vataru Nakamura6, João Carlos Palazzo de Mello6, Francisco Martins Teixeira2,7★★ Corresponding author

    1. 1Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Brazil.
    2. 2Laboratory of Research and Development in Pharmaceutical and Cosmetic, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
    3. 3College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Brazil.
    4. 4Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceição Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil.
    5. 5Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil.
    6. 6Pharmacy Course, Federal University of Rio de Janeiro, Campus Macaé, Av. Aluízio da Silva Gomes, 50 Granja dos Cavaleiros, CEP 27930-560, Macaé, Rio de Janeiro, Brazil.

    CORRESPONDENCE

    Francisco Martins Teixeira

    Laboratory of Research and Development in Pharmaceutical and Cosmetic, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.

    fteixeira@macae.ufrj.br

    Received: 31-03-2015; Revised: 01-07-2015.

    Volume 12, Issue 46s · pp. S218–S222 · PUBLISHED 11 May 2016 · DOI: 10.4103/0973-1296.182150

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Caries and periodontal diseases remain as important diseases in the Brazilian population. One important pathogen associated with this situation is Streptococcus mutans and other important factor is this pathogen’s ability to adhere firmly to the tooth surface leading to dental biofilm formation and caries development. Objectives: Determine the antibacterial and other biological activities of P. venusta related to its potential to be used in the treatment of caries and periodontal disease. Methods: The growth inhibition by P. venusta of Streptococcus mutans, S. mitis, S. oralis and Candida albicans was determined using the broth microdilution method. In addition, the effect of the samples in adherence and reducing production of acids by S. mutans, and germ-tube formation of C. albicans was analysed. The Nitric Oxide (NO) production and cytotoxicity of P. venusta to peripheral blood mononuclear cells (PBMC) and RAW 264.7 Cell Line Murine Macrophage from Blood were assessed. Results: The crude extract (CE) and ethyl-acetate (AF) and n-butanol (BF) fractions showed antibacterial activity. The ethyl-acetate (AF) fraction showed the highest inhibition percentage against the adherence of S. mutans and C. albicans cells without budding, beyond NO production inhibition. There was not any cytotoxicity in the murine macrophages RAW 264.7 cells. Conclusion: Our results suggest that P. venusta presents potential to be used as a preliminary source of compounds that can provide helpful activity when used in prophylaxis or treatment of caries or periodontal disease.

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      Sousa, M. B. D., Júnior, J. O. C. S., Barbosa, W. L. R., Valério, E. D. S., Lima, A. D. M., Araújo, M. H. D., Muzitano, M. F., Nakamura, C. V., Mello, J. C. P. D., & Teixeira, F. M. (2016). Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity. Pharmacognosy Magazine, 12(46s), S218–S222. https://doi.org/10.4103/0973-1296.182150