Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Optimization Extraction Process of Polysaccharides from Suillus granulatus and Their Antioxidant and Immunological Activities In vitro

    Feng Zhou1, Song Yan1, Shuang Chen1, Liying Gong1, Tingting Su1, Zhanyong Wang1★★ Corresponding author

    1. 1Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China, China.

    CORRESPONDENCE

    Zhanyong Wang

    Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China, China.

    wangzy125@lshu.edu.cn

    Received: 15-07-2015; Revised: 19-08-2015; Accepted: 19-08-2015.

    Volume 12, Issue 46s · pp. S277–84 · PUBLISHED 11 May 2016 · DOI: 10.4103/0973-1296.182161

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Suillus granulatus is an edible and medicinal fungus in China. S. granulatus polysaccharide (SGP) was considered as the main bioactivity compounds in S. granulatus. Therefore, the extraction of SGP and their antioxidant activities were studied in this work. Materials and Methods: Fruiting bodies of S. granulatus were purchased from a local market (Fushun, China). Response surface methodology was adopted to optimize the extraction conditions of SGP. The antioxidant and immunological activities in vitro were also assayed. Results: The extraction of SGP was optimized by a Box–Behnken design. The optimal conditions for the extraction of polysaccharides were as follows: Pre‑extraction time, 2 h; extraction temperature, 94°C; ratio of water to raw material, 25; and extraction frequency, 2. Under these conditions, the experimental yield of polysaccharides was 5.38% ±0.15%, which agreed with the predicted yield. The antioxidant assay in vitro showed that SGPs had relatively high scavenging ability for hydroxyl radicals and higher scavenging ability for 1,1‑diphenyl‑2‑picrylhydrazyl radical. However, the scavenging ability of SGPs for superoxide anion radical and reducing power was relatively low. The polysaccharides also significantly increased splenocyte proliferation in vitro. Conclusion: SGP possessed good antioxidant and immunological activities in vitro and explored as a novel natural antioxidant or functional food.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Zhou, F., Yan, S., Chen, S., Gong, L., Su, T., & Wang, Z. (2016). Optimization Extraction Process of Polysaccharides from Suillus granulatus and Their Antioxidant and Immunological Activities In vitro. Pharmacognosy Magazine, 12(46s), S277–84. https://doi.org/10.4103/0973-1296.182161