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    Essential Oil Composition, Antimicrobial and Pharmacological Activities of Lippia sidoides Cham. (Verbenaceae) From São Gonçalo do Abaeté, Minas Gerais, Brazil

    Sandra Ribeiro de Morais1,2, Thiago Levi Silva Oliveira1,2, Lanussy Porfiro de Oliveira3, Leonice Manrique Faustino Tresvenzol1, Edemilson Cardoso da Conceição1, Maria Helena Rezende4, Tatiana de Sousa Fiuza4, Elson Alves Costa3, Pedro Henrique Ferri5, José Realino de Paula1★★ Corresponding author

    1. 1Research Laboratory of Natural Products, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.
    2. 2Institute of Health Sciences, Paulista University, Campus Flamboyant, Goiânia, GO, Brazil.
    3. 3Institute of Biological Sciences, Department of Physiological Sciences, Federal University of Goiás, Goiânia, GO, Brazil.
    4. 4Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil.
    5. 5Institute of Chemistry, Molecular Bioactivity Laboratory, Federal University of Goiás, Goiânia, GO, Brazil.

    CORRESPONDENCE

    José Realino de Paula

    Research Laboratory of Natural Products, Faculty of Pharmacy, Federal University of Goiás, Goiânia, GO, Brazil.

    pjrpaula@gmail.com

    Received: 15-07-2015; Revised: 29-09-2015; Accepted: 29-09-2016.

    Volume 12, Issue 48 · pp. 262–270 · PUBLISHED 13 October 2016 · DOI: 10.4103/0973-1296.192197

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Lippia sidoides (Verbenaceae) is used in Brazilian folk medicine as an antiseptic, and it is usually applied topically on skin, mucous membranes, mouth, and throat, or used for vaginal washings. Objectives: To analyze the chemical composition of the essential oil from L. sidoides collected in São Gonçalo do Abaeté, Minas Gerais and grown in Hidrolândia, Goiás; to evaluate the antimicrobial activity of the essential oil, crude ethanol extract, and hexane, dichloromethane, ethyl-acetate, and aqueous fractions (AFs); to study the antinociceptive, anti-inflammatory, and central nervous system activities of the crude ethanol extract. Materials and methods: The essential oils were obtained by hydro-distillation using a Clevenger-type apparatus and analyzed by GC/MS. The antimicrobial activity in vitro was performed by broth microdilution method. The pharmacological tests were performed using female Swiss albino mice. Results: The major components of the essential oil were isoborneol (14.66%), bornyl acetate (11.86%), α-humulene (11.23%), α-fenchene (9.32%), and 1.8-cineole (7.05%), supporting the existence of two chemotypes of this species. The hexane fraction (HF) had good antifungal activity against Cryptococcus sp. ATCC D (MIC = 31.25 µg/mL) and Cryptococcus gatti L48 (MIC = 62.5 µg/mL). In the pharmacological tests, the crude ethanol extract presented antinociceptive and anti-inflammatory activities. Conclusion: Given that the ethanol extract of L. sidoides is included in the Formulary of Phytotherapeutic Agents of the Brazilian Pharmacopeia as an anti-inflammatory for oral cavities, the present work provides scientific evidence to back this use and highlight the importance of selecting the appropriate chemotype on the basis of the expected biological response.

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      Morais, S. R. D., Oliveira, T. L. S., Oliveira, L. P. D., Tresvenzol, L. M. F., Conceição, E. C. D., Rezende, M. H., Fiuza, T. D. S., Costa, E. A., Ferri, P. H., & Paula, J. R. D. (2016). Essential Oil Composition, Antimicrobial and Pharmacological Activities of Lippia sidoides Cham. (Verbenaceae) From São Gonçalo do Abaeté, Minas Gerais, Brazil. Pharmacognosy Magazine, 12(48), 262–270. https://doi.org/10.4103/0973-1296.192197