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    Article

    High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn

    Lu Zhao1, E Wen1, Halmuart Upur2, Shuge Tian3★★ Corresponding author

    1. 1College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, Xinjiang, China.
    2. 2College of TCM, Xinjiang Medical University, Urumqi, Xinjiang, China.
    3. 3Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.

    CORRESPONDENCE

    Shuge Tian

    Central Laboratory of Xinjiang Medical University, Urumqi, Xinjiang, China.

    tianshuge@hotmail.com

    Received: 08-10-2015; Revised: 03-11-2015; Accepted: 03-11-2015.

    Volume 13, Issue 49 · pp. 136–40 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197656

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Context: Sea buckthorn (Hippophae rhamnoides L.) as a traditional Chinese medicinal plant has various uses in Xinjiang. Objective: A reversed-phase rapid-resolution liquid-chromatography method with diode array detector was developed for simultaneous determination of protocatechuic acid, rutin, quercetin, kaempferol, and isorhamnetin in the pulp and seed of sea buckthorn, a widely used traditional Chinese medicine for promoting metabolism and treating scurvy and other diseases. Settings and design: Compounds were separated on an Agilent ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm; USA) with gradient elution using methanol and 0.4% phosphoric acid (v/v) at 1.0 mL/min. Detection wavelength was set at 280 nm. Materials and Methods: The fruits of wild sea buckthorn were collected from Wushi County in Aksu, Xinjiang Province. Statistical performances: The RSD of precision test of the five compounds were in the range of 0.60-2.22%, and the average recoveries ranged from 97.36% to 101.19%. Good linearity between specific chromatographic peak and component qualities were observed in the investigated ranges for all the analytes (R2 > 0.9997). Results: The proposed method was successfully applied to determine the levels of five active components in sea buckthorn samples from Aksu in Xinjiang. Conclusions: The proposed method is simple, fast, sensitive, accurate, and suitable for quantitative assessment of the pulp and seed of sea buckthorn.

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      Zhao, L., Wen, E., Upur, H., & Tian, S. (2017). High Performance Liquid Chromatography-Diode Array Detector Method for the Simultaneous Determination of Five Compounds in the Pulp and Seed of Sea Buckthorn. Pharmacognosy Magazine, 13(49), 136–40. https://doi.org/10.4103/0973-1296.197656