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    Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry

    Peng-peng Tian1,2, Xiao-xu Zhang1,2, Hong-ping Wang1, Pu-ling Li1,3, Yu-xin Liu2,4★, Shao-jing Li1★★ Corresponding author

    1. 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimen, Beijing, China.
    2. 2Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
    3. 3Henan University of Traditional Chinese Medicine, Zhengzhou, China.
    4. 4Drug Quality Control Key Laboratory of Hebei Province, Hebei University, Baoding, China.

    CORRESPONDENCE

    Yu-xin Liu

    Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, Baoding, China.

    yuxinliu@hbu.edu.cn

    Received: 01-11-2016; Revised: 05-12-2016; Accepted: 05-12-2016.

    Volume 13, Issue 49 · pp. 175–179 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197635

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Coptis chinensis Franch is a traditional Chinese medical herb. Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode. Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min. Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch.

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      Tian, P., Zhang, X., Wang, H., Li, P., Liu, Y., & Li, S. (2017). Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry. Pharmacognosy Magazine, 13(49), 175–179. https://doi.org/10.4103/0973-1296.197635