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    Antioxidant and Nephroprotective Activities of the Extract and Fractions of Homonoia riparia Lour

    Seena kanniparambil Xavier1, Shoja Muhammed Haneefa2, Devkar Raviraj Anand2, Picheswara Rao polo2, Rajalekshmi Maheshwari2, Chandrashekara Shastry Shreedhara2, Manganahalli Manjunath Setty2★★ Corresponding author

    1. 1Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
    2. 2Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.

    CORRESPONDENCE

    Manganahalli Manjunath Setty

    Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.

    mm.setty@manipal.edu

    Received: 15-06-2016; Revised: 11-07-2016; Accepted: 11-07-2016.

    Volume 13, Issue 49 · pp. 25–30 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197647

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Homonoia riparia is a plant, which is widely used in the indigenous system of medicine for the treatment of urolithiasis, renal disorders and inflammatory conditions. This is the first report on the antioxidant and nephroprotective activities of whole plant of H. riparia. Objective: The present study aims at investigating the in vitro antioxidant and nephroprotective activity of the methanol extract and its different fractions of H. riparia. Methods: Petroleum ether (HRPE), Ethyl acetate (HREA), Butanol (HRBU), aqueous fractions (HRAQ) were prepared from the crude methanol extract of H. riparia (HRM) using liquid partitioning. Total phenolic content, flavonoid content and antioxidant activity assay were performed according to suitable methods. Nephroprotective activities were evaluated by MTT assay using Human Embryonic Kidney cells against cisplatin induced toxicity. Quantification of gallic acid was performed using validated HPTLC method. Results: The studies showed that extract and fractions possess significant nephroprotective activity against cisplatin induced renal toxicity. All the extracts/fractions of whole plant of Homonoia riparia was found to be significantly reducing cisplatin induced toxicity (< 0.05). The highest activity was observed with HRBU and HRAQ with a percentage viability of 293.09 ± 4.3 and 345.07 ± 3.2 at a concentration of 200 µg/ml. Gallic acid was detected in the HRM/fractions using HPTLC.

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      Xavier, S. K., Haneefa, S. M., Anand, D. R., polo, P. R., Maheshwari, R., Shreedhara, C. S., & Setty, M. M. (2017). Antioxidant and Nephroprotective Activities of the Extract and Fractions of Homonoia riparia Lour. Pharmacognosy Magazine, 13(49), 25–30. https://doi.org/10.4103/0973-1296.197647