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    Article

    Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode

    Bang-Xing Han1,2, Yuan Yuan2, Lu-Qi Huang2, Qun Zhao1,2★, Ling-Ling Tan3, Xiang-Wen Song1, Xiao-Mei He1, Tao Xu4, Feng Liu1,4, Jian Wang4★ Corresponding author

    1. 1College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, Anhui Province, China.
    2. 2State Key Laboratory Breeding Base of Daodi Herbs, China Academy of Chinese Medical Sciences, Beijing, China.
    3. 3School of life science, Qingdao agricultural university, Qingdao, Shandong Province, China.
    4. 4Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.

    CORRESPONDENCE

    Qun Zhao

    College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an, Anhui Province, China.

    zhaoqun@hotmail.com

    Received: 19-12-2015; Revised: 20-01-2016; Accepted: 20-01-2016.

    Volume 13, Issue 49 · pp. 38–45 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197658

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The traditional Chinese medicine (TCM) Qianhu and Zihuaqianhu are the dried roots of Peucedanum praeruptorum and Angelica decursiva, respectively. Since the plant sources of Qianhu and Zihuaqianhu are more complex, the chemical compositions of P. praeruptorum and A. decursiva are significantly different, and many adulterants exist because of the differences in traditional understanding and medication habits. Therefore, the rapid and accurate identification methods are required. Objective: The aim was to study the feasibility of using DNA barcoding to distinguish between Traditional Chinese medicine Qianhu (Peucedanum praeruptorum), Zihuaqianhu (Angelica decursiva), and common adulterants, based on internal transcribed spacer (ITS) sequences, as well as specific PCR identification between P. praeruptorum and A. decursiva. Materials and Methods: The ITS sequences of P. praeruptorum, A. decursiva, and adulterant were studied, and a phylogenetic tree was constructed. Based on the ITS barcode, the specific PCR primer pairs QH-CP19s/QH-CP19a and ZHQH-CP3s/ZHQH-CP3a were designed for P. praeruptorum and A. decursiva, respectively. The amplification conditions were optimized, and specific PCR products were obtained. Results: The results showed that the phylogenetic trees constructed using the BI and MP methods were consistent, and P. praeruptorum and A. decursiva sequence haplotypes formed their own monophyly. The experimental results showed that in PCR products, the target bands appeared in the genuine drug and not in the adulterant, which suggests the high specificity of the two primer pairs. Conclusion: The ITS sequence was ideal DNA barcode to identify P. praeruptorum, A. decursiva, and adulterant. The specific PCR is a quick and effective method to distinguish between P. praeruptorum and A. decursiva.

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      Han, B., Yuan, Y., Huang, L., Zhao, Q., Tan, L., Song, X., He, X., Xu, T., Liu, F., & Wang, J. (2017). Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode. Pharmacognosy Magazine, 13(49), 38–45. https://doi.org/10.4103/0973-1296.197658