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    Pharmacognosy MagazineNOW IN
    Article

    Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes

    Seok Won Yoon1, Kang Pa Lee2, Do-Yoon Kim3, Dae Il Hwang3, Kyung-Jong Won3, Dae Won Lee3, Hwan Myung Lee3★★ Corresponding author

    1. 1Department of Cosmetic Science, College of Life and Health Sciences, Hoseo University, Asan, Chungnam Prefecture, Republic of Korea.
    2. 2Department of Physiology, School of Medicine, Konkuk University, Seoul city, Republic of Korea.
    3. 3Department of Bio-Science, Dongguk University, Gyeongju-city, Gyeongbuk Prefecture, Republic of Korea.

    CORRESPONDENCE

    Hwan Myung Lee

    Department of Bio-Science, Dongguk University, Gyeongju-city, Gyeongbuk Prefecture, Republic of Korea.

    kacsital@hoseo.edu

    Received: 13-04-2016; Revised: 24-06-2016; Accepted: 24-06-2016.

    Volume 13, Issue 49 · pp. 85–9 · PUBLISHED 6 January 2017 · DOI: 10.4103/0973-1296.197644

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Proliferation and migration of keratinocytes are essential for the repair of cutaneous wounds. Hibiscus syriacus L. has been used in Asian medicine; however, research on keratinocytes is inadequate. Objective: To establish the dermatological properties of absolute from Hibiscus syriacus L. flower (HSF) and to provide fundamental research for alternative medicine. Materials and Methods: We identified the composition of HSF absolute using gas chromatography-mass spectrometry analysis. We also examined the effect of HSF absolute in HaCaT cells using the XTT assay, Boyden chamber assay, sprout-out growth assay, and western blotting. We conducted an in-vivo wound healing assay in rat tail-skin. Results: Ten major active compounds were identified from HSF absolute. As determined by the XTT assay, Boyden chamber assay, and sprout-out growth assay results, HSF absolute exhibited similar effects as that of epidermal growth factor on the proliferation and migration patterns of keratinocytes (HaCaT cells), which were significantly increased after HSF absolute treatment. The expression levels of the phosphorylated signaling proteins relevant to proliferation, including extracellular signal-regulated kinase 1/2 (Erk 1/2) and Akt, were also determined by western blot analysis. Conclusion: These results of our in-vitro and ex-vivo studies indicate that HSF absolute induced cell growth and migration of HaCaT cells by phosphorylating both Erk 1/2 and Akt. Moreover, we confirmed the wound-healing effect of HSF on injury of the rat tail-skin. Therefore, our results suggest that HSF absolute is promising for use in cosmetics and alternative medicine.

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      Yoon, S. W., Lee, K. P., Kim, D., Hwang, D. I., Won, K., Lee, D. W., & Lee, H. M. (2017). Effect of Absolute From Hibiscus syriacus L. Flower on Wound Healing in Keratinocytes. Pharmacognosy Magazine, 13(49), 85–9. https://doi.org/10.4103/0973-1296.197644