Phytochemical and Biological Evaluations of Arum hygrophilum Boiss. (Araceae)
Fatma U. Afifi1★, Violet Kasabri1, Simona Litescu2, Ismail F. Abaza1, Khalid Tawaha1★ Corresponding author
- 1School of Pharmacy, The University of Jordan, Amman, Jordan.
- 2National Institute for Biological Sciences, Bucharest, Romania.
CORRESPONDENCE
Fatma U. Afifi
School of Pharmacy, The University of Jordan, Amman, Jordan.
Received: 28-03-2016; Revised: 29-04-2016; Accepted: 29-04-2016.
Volume 13, Issue 50 · pp. 275–80 · PUBLISHED 18 April 2017 · DOI: 10.4103/0973-1296.204551
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ABSTRACT
Background: Arum hygrophilum is a traditional medicinal plant indigenous to Jordan. The present study explores its phytochemistry, antioxidative, antidiabesity, and antiproliferative potentialities. Materials and Methods: Column chromatography and HPLC-MS analysis were used for its phytochemical evaluation. Using leaf crude water and ethanol extracts, the antioxidative capacities, their modulation of pancreatic β-cell proliferation, and insulin secretion as well as glucose diffusion and enzymatic bioassays were evaluated. Results: Three flavonoids (luteolin, isoorientin, and vitexin) and β-sitosterol have been isolated and their structures determined. HPLC-MS analysis of the ethanol extract further revealed the presence of caffeic, ferulic, gallic, and rosmarinic acids and quercetine-3-O-rhamnoside. The ethanol extract exhibited DPPH and ABTS radical scavenging and antioxidative capacities. A. hygrophilum (1), vitexin (2), and rosmarinic acid (3) inhibited pancreatic lipase (PL) dose dependently with PL-IC50 (µg/mL) values in an ascending order: (3); 51.28 ± 7.55 < (2); 260.9 ± 21.1 < (1); 1720 ± 10. Comparable to GLP-1-enhanced β-cell proliferation in 2-day treatment wells, a dose-dependent augmentation of BrdU incorporation was obtained with the A. hygrophilum aqueous extract (AE) (0.5 and 1 mg/mL, with respective 1.33- and 1.41-folds, P < 0.001). A. hygrophilum AE was identified as an inhibitor of α-amylase/α-glucosidase with IC50 value of 30.5 ± 2.1 mg/mL but lacked antiproliferative effects in colorectal cancer cell lines (HT29, HCT116, and SW620) and insulinotropic effects in β-cell line MIN6. Conclusion: A. hygrophilum extracts inhibited gastrointestinal enzymes involved in carbohydrate and lipid digestion and absorption.
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Afifi, F. U., Kasabri, V., Litescu, S., Abaza, I. F., & Tawaha, K. (2017). Phytochemical and Biological Evaluations of Arum hygrophilum Boiss. (Araceae). Pharmacognosy Magazine, 13(50), 275–80. https://doi.org/10.4103/0973-1296.204551