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    Effect of Helix aspersa extract on TNFα, NF-κB and some tumor suppressor genes in breast cancer cell line Hs578T

    Ibtissem Ouar1★, Cornelia Braicu2, Dalila Naimi3, Alexendru Irimie4, Ioana Berindan-Neagoe5★ Corresponding author

    1. 1Laboratory of Microbiological Engineering and Application, University Mentouri Constantine, Algeria.
    2. 2Department of Functional Genomics and Experimental Pathology, Cancer Institute “Ion Chiricuta”, Cluj-Napoca, Romania.
    3. 3Research Center for Functional Genomics, Biomedicine and Translational Medicine, Cluj-Napoca, Romania.
    4. 4Department of Surgery, Cluj-Napoca, Romania.
    5. 5Department of Surgical Oncology, Cluj-Napoca, Romania.
    6. 6Department of Immunology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.

    CORRESPONDENCE

    Ibtissem Ouar

    Ibtissem El Ouar,Laboratory of Microbiological Engineering and Application, University Mentouri Constantine, Algeria.

    ibtissem_elouar@yahoo.fr

    Received: 30-03-2015; Revised: 26-06-2015; Accepted: 18-04-2017.

    Volume 13, Issue 50 · pp. 281–5 · PUBLISHED 18 April 2017 · DOI: 10.4103/0973-1296.204618

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The garden snail, Helix aspersa, is a big land snail widely found in the Mediterranean countries. It is one of the most consumed species and widely used in zootherapy. Objective: The present study was carried out to investigate for the first time the first time the antitumor activity of an aqueous extract from Helix aspersa. Materials and Methods: The effect of H. aspersa extract was studied on a triple negative breast cancer cell line Hs578T. Firstly, the morphological changes and the mode of cell death induced by the extract have been evaluated by microscopy and acridine orange/ethidium bromide staining. The effect of the extract at dilution 0.1% and 1% was then tested on some genes, regulators of cell death and proliferation like tumor necrosis factor α (TNFα), NF- κB, and the tumor suppressor genes P53 and PTEN. Results: Data demonstrate that the extract induces necrosis in tumor cells. It enhances significantly the expression of TNFα; mRNA levels were 20 and 10 times more important in treated cells compared to nontreated cells. NF-κB and PTEN were inhibited with the dilution 1% after 8 and 24 hours of treatment. P53 expression was further inhibited but only with the highest dose, after 4, 8, and 24 hours. Conclusion: Our results show that H. aspersa extract has an antitumor activity against Hs578T cells; it is a potent stimulator for TNFα and a good inhibitor for NF-κB.

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      Ouar, I., Braicu, C., Naimi, D., Irimie, A., & Berindan-Neagoe, I. (2017). Effect of Helix aspersa extract on TNFα, NF-κB and some tumor suppressor genes in breast cancer cell line Hs578T. Pharmacognosy Magazine, 13(50), 281–5. https://doi.org/10.4103/0973-1296.204618