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    Development and Validation of a Simultaneous RP-HPLC-UV/DAD Method for Determination of Polyphenols in Gels Containing S. terebinthifolius Raddi (Anacardiaceae)

    Melina G. Carvalho1, Cícero F. S Aragão2, Fernanda N. Raffin2, Túlio F. A. de L. Moura2★★ Corresponding author

    1. 1Programa de Pós-graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Norte-UFRN, Natal, RN, Brasil.
    2. 2Departamento de Farmácia da Universidade Federal do Rio Grande do Norte - UFRN, Brasil.

    CORRESPONDENCE

    Túlio F. A. de L. Moura

    Departamento de Farmácia da Universidade Federal do Rio Grande do Norte - UFRN, Brasil.

    moura@ufrnet.br

    Received: 15-07-2015; Revised: 03-09-2015; Accepted: 18-04-2017.

    Volume 13, Issue 50 · pp. 309–15 · PUBLISHED 18 April 2017 · DOI: 10.4103/0973-1296.204559

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Topical gels containing extracts of Schinus terebinthifolius have been used to treat bacterial vaginosis. It has been reported that this species has anti-microbial, anti-inflammatory and anti-ulcerogenic properties, which can be attributed to the presence of phenolic compounds. In this work, a sensitive and selective reversed-phase HPLC-UV/DAD method for the simultaneous assay of six polyphenols that could be present in S. terebinthifolius was developed. The method was shown to be accurate and precise. Peak purity and similarity index both exceeded 0.99. Calibration curves were linear over the concentration range studied, with correlation coefficients between 0.9931 and 0.9974. This method was used to determine the polyphenol content of a hydroalcoholic extract and pharmacy-compounded vaginal gel. Although the method is useful to assess the 6 phenolic compounds, some compounds could not be detected in the products.

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      Carvalho, M. G., Aragão, C. F. S., Raffin, F. N., & Moura, T. F. A. D. L. (2017). Development and Validation of a Simultaneous RP-HPLC-UV/DAD Method for Determination of Polyphenols in Gels Containing S. terebinthifolius Raddi (Anacardiaceae). Pharmacognosy Magazine, 13(50), 309–15. https://doi.org/10.4103/0973-1296.204559