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    Article

    Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) Manden and Scheng

    Abdussamat Guzel1, Huseyin Aksit1, Mahfuz Elmastas1, Ramazan Erenler1★★ Corresponding author

    1. 1Department of Chemistry, Faculty of Science and Art, Gaziosmanpasa University, Tokat, Turkey.

    CORRESPONDENCE

    Ramazan Erenler

    Department of Chemistry, Faculty of Science and Art, Gaziosmanpasa University, Tokat, Turkey.

    rerenler@gmail.com

    Received: 07-06-2016; Revised: 22-07-2016; Accepted: 22-07-2016.

    Volume 13, Issue 50 · pp. 316–320 · PUBLISHED 18 April 2017 · DOI: 10.4103/0973-1296.204556

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Medicinal and aromatic plants play a significant role in drug discovery and development process. Flavonoids, revealing a wide spectrum of biological activities, extensively found in plants are important secondary metabolites. Materials and Methods: Aerial parts of Cyclotrichium origanifolium were collected, dried, and boiled in water then extracted with hexane, ethyl acetate, and n-butanol. Total phenolic content, DPPH• scavenging activity, reducing power (FRAP) activity, and ABTS•+ scavenging activity assays were applied for all extracts. The ethyl acetate extract revealing the most antioxidant activity as well as including the highest phenolic contents was subjected to chromatographic techniques (column chromatography, sephadex LH-20, semipreparative HPLC) to isolate the active compounds. The structure of isolated compounds were elucidated by spectroscopic methods (1D NMR, 2D NMR, and LC-TOF/MS). Results: Isosakuranetin (1), eriodictyol (2), luteolin (3), naringenin (4), and apigenin (5) were isolated and identified. All isolated flavonoids displayed the excellent antioxidant activity. Conclusion: The isolated flavonoids and also plant extract have potency to be a natural antioxidant.

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      Guzel, A., Aksit, H., Elmastas, M., & Erenler, R. (2017). Bioassay-guided isolation and identification of antioxidant flavonoids from Cyclotrichium origanifolium (Labill.) Manden and Scheng. Pharmacognosy Magazine, 13(50), 316–320. https://doi.org/10.4103/0973-1296.204556