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    Antiurolithiatic Potential of Neeri against Calcium‑Oxalate Stones by Crystallization Inhibition, Free Radicals Scavenging, and NRK‑52E Cell Protection from Oxalate Injury

    Parveen Kumar Goyal2,3, Santosh Kumar Verma4, Anil Kumar Sharma5★★ Corresponding author

    1. 1Research Scholar, I.K.G. Punjab Technical University, Kapurthala, Punjab, India.
    2. 2Department of Pharmacology, Hindu College of Pharmacy, Sonepat, Haryana, India.
    3. 3Department of Pharmacology, Motherhood University, Roorkee, Uttrakhand, India.
    4. 4Department of Pharmacognosy, CT Institute of Pharmaceutical Sciences, Jalandhar, Punjab, India.

    CORRESPONDENCE

    Anil Kumar Sharma

    aksharma91@gmail.com

    Received: 06-12-2016; Revised: 23-01-2017.

    Volume 13, Issue 51s · pp. S549–S554 · PUBLISHED 11 October 2017 · DOI: 10.4103/pm.pm_551_16

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Neeri is a well‑established polyherbal formulation prescribed for renal stones by the physicians but has not been experimentally evaluated for its antiurolithiatic potential using cell‑lines. Objective: This study is aimed to scientifically substantiate the antiurolithiatic effect of Neeri extract (NRE) through calcium oxalate (CaOx) crystallization inhibition, scavenging of free radicals, and protection of renal tubular epithelial NRK‑52E cells from oxalate‑induced injury. Materials and Methods: The crystallization inhibition was studied by turbidimetric assay while the free radical scavenging potential was determined for superoxide and nitric oxide (NO) radicals. The cytoprotective effect against oxalate‑induced injury was assessed by estimating lactate dehydrogenase (LDH) leakage and determining cell viability using 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. Results: NRE significantly inhibited the CaOx crystallization in a concentration‑dependent manner and also scavenged superoxide (IC50 302.88 µg/ml) and NO (IC50 300.45 µg/ml) free radicals. It did not show any significant cytotoxicity for NRK‑52E cells till the highest dose (500 µg/ml) and found to be safe. When NRK‑52E cells, injured by exposing to oxalate crystals for 24 h, were treated with NRE, it appreciably prevented the cell injury in a dose‑dependent manner. It significantly decreased the elevated LDH leakage toward normal range and improved renal cell viability (82.37% ± 0.87%), hence, prevented growth and retention of crystals. Conclusion: The experimental findings concluded that Neeri is a potent antiurolithiatic formulation that inhibited CaOx crystallization and prevented tubular retention of crystals by protecting the renal cells against oxalate‑induced injury as well as reducing the oxidative stress by scavenging free radicals.

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      Goyal, P. K., Verma, S. K., & Sharma, A. K. (2017). Antiurolithiatic Potential of Neeri against Calcium‑Oxalate Stones by Crystallization Inhibition, Free Radicals Scavenging, and NRK‑52E Cell Protection from Oxalate Injury. Pharmacognosy Magazine, 13(51s), S549–S554. https://doi.org/10.4103/pm.pm_551_16