Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Methyl Jasmonate and Salicylic Acid Enhanced the Production of Ursolic and Oleanolic Acid in Callus Cultures of Lepechinia Caulescens

    Víctor M. Vergara Martínez1, Samuel E. Estrada-Soto2, José de Jesús Arellano-García1, Julio C. Rivera-Leyva2, Patricia Castillo-España1, Angélica Flores Flores2, Alexandre T. Cardoso-Taketa1, Irene Perea-Arango1★★ Corresponding author

    1. 1Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.
    2. 2Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.

    CORRESPONDENCE

    Irene Perea-Arango

    Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, México.

    iperea@uaem.mx

    Received: 06-03-2017; Revised: 27-04-2017; Accepted: 31-01-2018.

    Volume 13, Issue 52s · pp. S886–9 · PUBLISHED 31 January 2018 · DOI: 10.4103/pm.pm_77_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The production of triterpenes from plants for pharmacological purposes varies in concentration, due to genetic and environmental factors. In vitro culture enables the control and increase of these bioactive molecules. Objective: To evaluate the effect of plant growth regulators and elicitors in the induction of calli and the production of ursolic acid (UA) and oleanolic acid (OA) in Lepechinia caulescens. Materials and Methods: Leaf explants were exposed for the induction of calli at different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). Methyl jasmonate (MJ) and salicylic acid were used as elicitors. High-performance liquid chromatography method was used to quantify UA and OA content in each treatment. Results: Treatment with 3.0 mg/L of 2,4-D and 0.1 mg/L of BAP produced the best results for calli induction and production of UA (1.57 mg/g dry weight [DW]) and OA (1.13 mg/g DW). Both elicitors facilitated the accumulation of triterpenes. Conclusion: The combination of auxins and cytokinins showed favorable results for the induction of calli. Variation concerning the accumulation of UA and OA was observed between treatments. MJ increased the production of triterpenes five times after 8 h of exposure, compared to control treatment. There is a greater accumulation of UA (16.58 mg/g DW) and OA (1.94 mg/g DW) in leaves of wild plants.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Martínez, V. M. V., Estrada-Soto, S. E., Arellano-García, J. D. J., Rivera-Leyva, J. C., Castillo-España, P., Flores, A. F., Cardoso-Taketa, A. T., & Perea-Arango, I. (2018). Methyl Jasmonate and Salicylic Acid Enhanced the Production of Ursolic and Oleanolic Acid in Callus Cultures of Lepechinia Caulescens. Pharmacognosy Magazine, 13(52s), S886–9. https://doi.org/10.4103/pm.pm_77_17