Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Modeling and Optimum Extraction of Multiple Bioactive Exopolysaccharide from an Endophytic Fungus of Crocus sativus L

    Lu Wen1, Yuan Xu1, Qiqiu Wei1, Wuhai Chen1, Gang Chen1★★ Corresponding author

    1. 1Departments of Medicinal Chemistry and Pharmaceutics, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.

    CORRESPONDENCE

    Gang Chen

    Departments of Medicinal Chemistry and Pharmaceutics, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China.

    cg753@126.com

    Received: 09-03-2017; Revised: 27-04-2017; Accepted: 20-02-2018.

    Volume 14, Issue 53 · pp. 36–43 · PUBLISHED 20 February 2018 · DOI: 10.4103/pm.pm_96_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Crocus sativus L. (saffron) is a scarce plant that has been used as food flavoring agent, coloring agent, and traditional herbal medicine. Methods: The bioactivity of exopolysaccharide (EPS) extracted from an endophytic fungus of C. sativus was examined for the first time by antioxidative, antitumor, and antibacterial assays. The extraction conditions for EPS were optimized by combining the response surface methodology with Box‑Behnken design. Results: EPS exhibited excellent scavenging activities against 1,1‑diphenyl‑2‑picrylhydrazyl, hydroxyl and superoxide anion radicals, and moderate cytotoxicities against K562, A549, HL‑60, and HeLa cells. The optimum extraction conditions for EPS were as follows: precipitation time of 16 h, precipitation temperature of 3.7°C, pH 7.2, and ratio of ethanol to fermented broth of 5:1 (L/L). Under the optimized conditions, the yield of EPS reached 162 ± 6 μg/L which was close to the predicted one (165 μg/L). Moreover, high‑performance liquid chromatography of monosaccharide composition showed that EPS comprised mannose, glucose, galactose xylose, and arabinose in a molar ratio of 25.6:16.5:1.0:3.8:5.4. Conclusion: EPS may be an eligible substitute for C. sativus and a potential bioactive source applicable to pharmaceutical and food industries.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Wen, L., Xu, Y., Wei, Q., Chen, W., & Chen, G. (2018). Modeling and Optimum Extraction of Multiple Bioactive Exopolysaccharide from an Endophytic Fungus of Crocus sativus L. Pharmacognosy Magazine, 14(53), 36–43. https://doi.org/10.4103/pm.pm_96_17