Efficient Preparation of Narcissin from Opuntia ficus‑indica Fruits by Combination of Response Surface Methodology and High‑speed Countercurrent Chromatography
Guijae Yoo1, Youngse Oh1, Heejung Yang2, Tae Bum Kim3, Sang Hyun Sung3, Seung Hyun Kim1★★ Corresponding author
- 1College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Korea.
- 2College of Pharmacy, Kangwon National University, Chunchon, Korea.
- 3College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea.
CORRESPONDENCE
Seung Hyun Kim
College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, Korea.
Received: 12-09-2017; Revised: 19-10-2017.
Volume 14, Issue 56 · pp. 338–343 · PUBLISHED 14 August 2018 · DOI: 10.4103/pm.pm_422_17
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ABSTRACT
Background: Narcissin is well known for their various biological activities. Objective: To recover narcissin from the Opuntia ficus‑indica fruits (OFIF), an efficient method was developed by a combination of response surface methodology (RSM) and high‑speed countercurrent chromatography (HSCCC). Materials and Methods: Optimization of extraction conditions of narcissin from OFIF was determined using RSM with three‑level‑three‑factor Box‑Behnken design (BBD). Then, a rapid and efficient method for the isolation of narcissin from the rich narcissin extracts was developed using HSCCC. Results: Regression analysis showed a good fit of the experimental data and the optimal condition was obtained as extraction time (X1), 6.02 h; solvent to material ratio (X2), 8.16 mL/g; and ethanol concentration (X3), 93.48%. Then, the rich narcissin extracts were separated by HSCCC with a two‑phase solvent system composed of n‑hexane: ethyl acetate: methanol: water (1.5:5:5:1.5, v/v/v/v) in one step within 60 min. As a result, 12 mg of narcissin was isolated from 100 mg of crude extract with purities of 98.5%, as determined by high‑performance liquid chromatography (HPLC). Conclusion: This study can be useful to the development of industrial extraction processes, including further studies concerning the optimal number of sequential steps to enhance the efficacy of a large‑scale extraction system.
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Yoo, G., Oh, Y., Yang, H., Kim, T. B., Sung, S. H., & Kim, S. H. (2018). Efficient Preparation of Narcissin from Opuntia ficus‑indica Fruits by Combination of Response Surface Methodology and High‑speed Countercurrent Chromatography. Pharmacognosy Magazine, 14(56), 338–343. https://doi.org/10.4103/pm.pm_422_17