Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Flavonoid and Phenolic Compounds from Carissa macrocarpa:

    Molecular Docking1, Cytotoxicity Studies Hany E. Khalil2,3★, Maged E. Mohamed2,4, Mohamed A. Morsy2,5, Mahmoud Kandeel6,7★ Corresponding author

    1. 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University.
    2. 2Department of Pharmacognosy, Faculty of Pharmacy, Minia University.
    3. 3Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig.
    4. 4Department of Pharmacology, Faculty of Medicine, Minia University, Minia.
    5. 5Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, King Faisal University, Al‑Ahsa, Saudi Arabia.
    6. 6Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El‑Sheikh, Egypt.

    CORRESPONDENCE

    Cytotoxicity Studies Hany E. Khalil

    Department of Pharmacognosy, Faculty of Pharmacy, Minia University.

    heahmed@kfu.edu.sa

    Received: 12-03-2018; Revised: 11-04-2018.

    Volume 14, Issue 57s · pp. S304–S310 · PUBLISHED 10 September 2018 · DOI: 10.4103/pm.pm_104_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The objective of the study is to investigate the phytochemical contents of the methanol extract of leaves of Carissa macrocarpa, the possible anticancer activities of the isolated compounds through molecular docking approaches as well as the potential cytotoxic activity. Materials and Methods: The methanol extract of the plant was subjected to several chromatographic procedures. In silico studies of the isolated compounds against four anticancer target kinases, namely, protein kinase B (PKB/AKT), phosphatidylinositol 3‑kinase, protein kinase C, and rapidly accelerated fibrosarcoma kinase were performed. Potential cytotoxic activity of the isolated compounds was determined by 3‑(4,5‑dimethyl‑2‑thiazolyl)‑2,5‑diphenyl‑2H‑tetrazolium bromide assay against A549 cells. Results: Phytochemical investigation led to the isolation of three known flavonoid compounds: kaempferol 3‑O‑robinobioside (1), Kaempferol‑3‑O‑α‑L‑rhamnopyranosyl (1‑6)(4``‑p‑coumaroyl)β‑D‑galac topyranoside7‑O‑α‑L‑rhamnopyranoside (2), and variabiloside E (3) as well as three phenolic compounds: p‑coumaric acid (4), salicin (5), and 3,4‑dimethylphenol β‑gentiobioside (6). In silico studies revealed that three out of the six compounds were strongly bound with one or more of the targets enzymes. Compound 3 showed broad‑spectrum binding with the four targets with high docking score. Compounds 1–3 showed IC50 comparable to that of positive control, doxorubicin. The rest of the compounds 4–6 showed relatively discrete IC50. Conclusion: The isolated compounds were reported for the first time from this plant. Compounds 1–3 could serve as lead compounds for development of new anticancer drugs.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Docking, M., Khalil, C. S. H. E., Mohamed, M. E., Morsy, M. A., & Kandeel, M. (2018). Flavonoid and Phenolic Compounds from Carissa macrocarpa:. Pharmacognosy Magazine, 14(57s), S304–S310. https://doi.org/10.4103/pm.pm_104_18