Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Hepatic Immune Response to Environmental Carcinogens

    Kawa Amin2,3★, Kosar Muhammad Ali4, Amanj Saeed5, Heshu Sulaiman Rahman6, Jonas Bystrom7★ Corresponding author

    1. 1Department of Medical Science, Respiratory Medicine and Allergology, Clinical Chemistry and Asthma Research Centre, Uppsala University and University Hospital, Uppsala, Sweden.
    2. 2Departments of Microbiology/Immunology and, University and University Hospital, Uppsala, Sweden.
    3. 3Medicine, College of Medicine, University of Sulaimani, Sulaimani.
    4. 4Ministry of Higher Education and Scientific Research, Erbil.
    5. 5College of Veterinary Medicine, University of Sulaimani, Sulaimani, Iraq.
    6. 6BQ, UK.

    CORRESPONDENCE

    Kawa Amin

    Departments of Microbiology/Immunology and, University and University Hospital, Uppsala, Sweden.

    kawa.amin@medsci.uu.se

    Received: 10-05-2018; Revised: 22-06-2018.

    Volume 14, Issue 58 · pp. 548–553 · PUBLISHED 21 November 2018 · DOI: 10.4103/pm.pm_242_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Aim: Environmental carcinogenic substances contribute to increasing incidence of hepatocellular carcinoma (HCC). We employed a sensitive method for the detection of DNA damage combined with analysis of the immune response to gain better knowledge how environmental carcinogens mediate pathology. Materials and Methods: Rat hepatocytes were isolated and stimulated with carcinogenic substances for the assessment of DNA damage. The mycotoxin aflatoxin B1 (AFB1), two heterocyclic amines from the cooking of meat amino‑3‑methylimidazo[4,5‑f] quinoline (IQ) and 3‑amino‑1‑methyl‑5H‑pyr ido‑(4,3‑b)‑indole (TRP‑P‑2), and protein extract from the fungus Lactarius necator were assayed. Unscheduled DNA synthesis in hepatocytes was measured by the incorporation of radioactive thymidine during DNA repair. Stimulation of hepatocyte/immune cell preparation with the substances and measurement of IFNγ release at different time points determined their ability to induce an inflammatory response. Results: DNA repair in the hepatocytes was induced in response to 10−7 M AFB1 and 10−9 M IQ. TRP‑P‑2 did not induce DNA repair; however, at 10−4 M, the fungus extract did this. Furthermore, liver‑resident immune cells responded with differential production of IFNγ over time in response to stimulation by all the carcinogens, with AFB1 being the most potent. TRP‑P‑2 showed the most significant reduction in IFNγ response over time. Conclusion: DNA damage in hepatocytes induced by environmental substances was detected at low molecular concentrations. The system did provide novel evidence for hepatic carcinogenicity by the fungus L. necator. Analysis of the response by liver‑resident immune cells to the substances suggested that highly mutagenic substances induce prolonged inflammatory response.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Amin, K., Ali, K. M., Saeed, A., Rahman, H. S., & Bystrom, J. (2018). Hepatic Immune Response to Environmental Carcinogens. Pharmacognosy Magazine, 14(58), 548–553. https://doi.org/10.4103/pm.pm_242_18