Systematic Understanding the Mechanisms of Tripterygium wilfordii on Atherosclerosis and Pharmacodynamics Research in Apo E‑/‑mice Model
Jingyan Liang2,3,4★, Lu Chen2★, Yang Pan2★, Yayun Qian2,3, Lifu Wei2, Yumeng Zhang2, Kaiming He2, Yanqing Liu2,3, Yingge Wang2,3,4,5★★ Corresponding author
- 1Research Center for Vascular Biology, School of Medicine, Yangzhou University.
- 2Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou University.
- 3Jiangsu Co‑Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University.
- 4Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China ORIGINAL ARTICLE.
CORRESPONDENCE
Jingyan Liang
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Disease, Yangzhou University.
Received: 27-11-2017; Revised: 10-01-2018.
Volume 14, Issue 58 · pp. 624–633 · PUBLISHED 21 November 2018 · DOI: 10.4103/pm.pm_556_17
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ABSTRACT
Background: Atherosclerosis (AS) is a chronic arterial disease and a major cause of vascular death, with multiple pathogenesis including chronic inflammatory. Tripterygium wilfordii (TGW) had a good effect on an anti‑inflammatory. At present, more and more researches indicated that TGW could also regulate AS. Objective: The aim of this study is to clarify what the anti‑atherosclerotic ingredients are in TGW and whether these ingredients improve AS synergistically. Materials and Methods: First, systematic pharmacology was utilized to predict the active ingredients and potential targets of TGW related to AS. Then, a bioactive compound of triptolide (TPL) and Tripterine (TPR) in TGW were evaluated if they presented the synergistically anti‑atherosclerotic effects in Apo E‑/‑mice fed with a high‑fat/high‑cholesterol diet. In the experiment, Hematoxylin and Eosin tested the plaque areas; reverse transcriptase‑ polymerase chain reaction and Western blot analysis detected the matrix metalloprotein 9 (MMP‑9), tumor necrosis factor alpha (TNF‑α), and NF‑κB levels in the aortas. Results: The results shown that there are 17 bioactive compounds with 76 therapeutic proteins were identified. Moreover, TGW exhibits a protective effect on treatment AS likely through regulating multiple pathways including immune response, inflammatory response, and vascular structure improving. Further verified that TPL combined with TPR in TGW had synergistic effect on treatment AS by reducing levels of MMP‑9, TNF‑α, and NF‑κB, might be the important pathway. Conclusion: TGW, synergistic effect of different compounds, could regulate AS by multiple pathways, especially improving immune response, inflammatory response, and vascular structure. The major compounds of Tripterine and Triptolide in TGW had a synergistic effect on anti‑AS by suppressing matrix metalloprotein 9, TNF‑α, and NF‑κB.
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Liang, J., Chen, L., Pan, Y., Qian, Y., Wei, L., Zhang, Y., He, K., Liu, Y., & Wang, Y. (2018). Systematic Understanding the Mechanisms of Tripterygium wilfordii on Atherosclerosis and Pharmacodynamics Research in Apo E‑/‑mice Model. Pharmacognosy Magazine, 14(58), 624–633. https://doi.org/10.4103/pm.pm_556_17