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    Characterization of the Secondary Metabolites from Endophytic Fungi Nodulisporium sp. Isolated from the Medicinal Plant Mikania laevigata (Asteraceae) by Reversed‑Phase High‑Performance Liquid Chromatography Coupled with Mass Spectrometric Multistage

    Isabella M. A. Reis1, Fernanda P. C. Ribeiro1, Paulo R. M. Almeida2, Larissa C. B. Costa3, Hélio M. Kamida2, Ana P. T. Uetanabaro3, Alexsandro Branco1★★ Corresponding author

    1. 1Departments of Health, University of Feira de Santana, Feira de Santana, Brazil.
    2. 2Biology, State University of Feira de Santana, Feira de Santana, Brazil.
    3. 3Department of Biological Sciences, State University of Santa Cruz, Ilheus, BA, Brazil.

    CORRESPONDENCE

    Alexsandro Branco

    Departments of Health, University of Feira de Santana, Feira de Santana, Brazil.

    branco@uefs.br

    Received: 22-12-2017; Revised: 28-02-2018.

    Volume 14, Issue 59s · pp. S495–S498 · PUBLISHED 17 January 2019 · DOI: 10.4103/pm.pm_616_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The endophytic fungi are an excellent source of secondary metabolites as a natural product with antimicrobial, insecticidal, cytotoxic, antioxidant, and anticancer activities. No studies on the chemical compounds of the endophytes of Mikania laevigata have been described in the literature. Objective: The objective of the study is to investigate by reversed phase high-performance liquid chromatography coupled with mass spectrometric multistage (RP-HPLC-MS/MS) the secondary metabolites of the endophytic fungi Nodulisporium sp., isolated from the medicinal plant M. laevigata (Asteraceae). Materials and Methods: The mycelia biomass strains of Nodulisporium sp. were accumulated and separated from the aqueous medium by filtration; the filtrate was subjected to a liquid–liquid partition with ethyl acetate (EtOAc) resulting in the crude EtOAc extract. This extract was analyzed by RP-HPLC-MS/MS. Results: The total mass chromatogram of the EtOAc crude strain Nodulisporium sp. extract showed compounds eluted between 13 and 20 min. The peak 1 (Rt 13.8 min), peak 2 (Rt. 14.8 min), peak 3 (Rt. 15.7 min) and peak 4 (Rt. 19.6 min) were characterized as dechlorogriseofulvin (C17H18O6), griseofulvin (C17H17O6Cl), cytochalasin D (C30H37NO6) and nodulisporic acid B2 (C43H57NO7), respectively. Conclusion: The present work provides the first scientific report on constituents of endophytic fungi Nodulisporium sp. isolated from the M. laevigata (Asteraceae).

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      Reis, I. M. A., Ribeiro, F. P. C., Almeida, P. R. M., Costa, L. C. B., Kamida, H. M., Uetanabaro, A. P. T., & Branco, A. (2019). Characterization of the Secondary Metabolites from Endophytic Fungi Nodulisporium sp. Isolated from the Medicinal Plant Mikania laevigata (Asteraceae) by Reversed‑Phase High‑Performance Liquid Chromatography Coupled with Mass Spectrometric Multistage. Pharmacognosy Magazine, 14(59s), S495–S498. https://doi.org/10.4103/pm.pm_616_17