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    Picrolemma sprucei Quassinosides Inhibits Breast and Prostate Cell Growth and Impairs Behavioral Phenotype in Mice

    Sinária Rejany Nogaia de Sousa2, Ingrit Elida Collantes Díaz3, Riad Naim Younes4, Sergio Alexandre Frana5, Maria Martha Bernardi2, Ivana Barbosa Suffredini2,4★★ Corresponding author

    1. 1Graduate Program in Environmental and Experimental Pathology, Paulista University.
    2. 2Departamento de Ingeniería Química, Faculdad de Ingeniería Química y Textil, Universidad Nacional de Ingeniería, Lima, Perú.
    3. 3Center for Research in Biodiversity, Extraction Laboratory, Paulista University.
    4. 4Center for Research in Biodiversity, Botany Laboratory and Herbarium UNIP, Paulista University, São Paulo, Brazil.

    CORRESPONDENCE

    Ivana Barbosa Suffredini

    Departamento de Ingeniería Química, Faculdad de Ingeniería Química y Textil, Universidad Nacional de Ingeniería, Lima, Perú.

    ibsuffredini@yahoo.com.br

    Received: 09-05-2018; Revised: 28-06-2018.

    Volume 15, Issue 60 · pp. 1–11 · PUBLISHED 23 January 2019 · DOI: 10.4103/pm.pm_228_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The objective of the study is to bioguide fractionate the organic/aqueous extracts from the aerial organs of Picrolemma sprucei (EB1151 and EB1152) aiming the identification of antitumor compounds and to evaluate the influence of EB1151 on mice behavioral phenotype. Materials and Methods: The breast (MCF‑7) and prostate (PC‑3) cancer cell lines cytotoxicity assay were assessed using the sulforhodamine B method. Fractionation was carried out by thin layer and column chromatographic techniques. The influence of the intraperitoneal administration of nonlethal dose (NLD, 39.1 mg/kg) of EB1151 to Balb‑c male mice was assessed in open cage and open‑field (OF) apparatuses. Results: FCHCl3 partition phases from EB1151 and EB1152 showed cytotoxic activity against both cancer cells, as did fractions UNIP343, which contains sergeolide and isobruceine B, and UNIP344, which contains sergeolide and 15‑deacetylsergeolide. Higher doses of EB1151 showed significant alterations in response to touch, hindquarter fall, irritability, auricular and corneal reflexes, ataxia, micturition, ptosis, piloerection, hypothermia, and cyanosis, and NLD showed alterations in general activity and response to touch, but recovery was obtained up to the end of the experiment. Conclusions: Quassinosides from the aerial organs of P. sprucei showed significant cytotoxicity against MCF‑7 and PC‑3 cell lines. Extract EB1151 has impaired general activity and activity in the OF apparatus, but recovery was observed.

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      Sousa, S. R. N. D., Díaz, I. E. C., Younes, R. N., Frana, S. A., Bernardi, M. M., & Suffredini, I. B. (2019). Picrolemma sprucei Quassinosides Inhibits Breast and Prostate Cell Growth and Impairs Behavioral Phenotype in Mice. Pharmacognosy Magazine, 15(60), 1–11. https://doi.org/10.4103/pm.pm_228_18