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    Pharmacokinetic Study of Hepatoprotective Coumarinolignoids from Cleome viscosa in Mice Using Validated High‑Performance Liquid Chromatography‑Photodiode Array Method

    Kuldeep Singh Yadav1, Ranjana Ranjana2, Sudeep Tandon3, Narayan Prasad Yadav1★, Karuna Shanker2★★ Corresponding author

    1. 1Departments of Botany and Pharmacognosy, Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.
    2. 2Analytical Chemistry and, India.
    3. 3Process Chemistry and Chemical Engineering, CSIR‑Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.

    CORRESPONDENCE

    Narayan Prasad Yadav

    Departments of Botany and Pharmacognosy, Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.

    np.yadav@cimap.res.in

    Received: 04-10-2018; Revised: 13-11-2018.

    Volume 15, Issue 61 · pp. 270–276 · PUBLISHED 6 March 2019 · DOI: 10.4103/pm.pm_508_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: However, the bioavailability and pharmacokinetics of Cliv‑92 are still not clear. The present study is the first validated method which deals with the assay of Cliv‑92 in mouse plasma. Methods: Single‑step sample preparation meets the criteria of recovery (80%–93% with relative standard deviation [RSD] 2.14%–4.80%). Reverse‑phase high‑performance liquid chromatography with photodiode array detection has resulted into acceptable separation and sensitivity of three structurally similar cleomiscosins – A, B, and C of Cliv‑92. The analysis involved a binary gradient of mobile phase and flow rate. Quantification was done at peak area at 326 nm using linear regression curve (r2 > 0.999). The precision (0.46%–2.68% RSD) and accuracy (±2.09% bias) of Cliv‑92 determination in plasma complied the criteria of the current international guidelines. We have also evaluated the matrix effect on sensitivities by spiking method. Limit of detection and limit of quantification in mouse plasma ranged between 0.13–0.24 µg/ml and 0.41–0.74 µg/ml. Results: Pharmacokinetic parameters were studied after intravenous bolus administration of Cliv‑92 at 10 mg/kg dose in mice. Blood samples were collected at a predefined time up to 24 h post-injection. The Cliv‑92 plasma half‑life (t1/2) was 2.77 h, and the clearance was estimated as 2.38 L/h/kg. Conclusion: The method is simple, sensitive, and accurate for the determination of plasma concentration of coumarinolignoids. The present preclinical pharmacokinetic study of coumarinolignoids has been anticipated in clinical studies with scaling techniques.

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      Yadav, K. S., Ranjana, R., Tandon, S., Yadav, N. P., & Shanker, K. (2019). Pharmacokinetic Study of Hepatoprotective Coumarinolignoids from Cleome viscosa in Mice Using Validated High‑Performance Liquid Chromatography‑Photodiode Array Method. Pharmacognosy Magazine, 15(61), 270–276. https://doi.org/10.4103/pm.pm_508_18