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    Article

    Effect of Gardenia/Acanthopanax‑Medicated Serum on High Level Corticosterone‑induced Pheochromocytoma Cell Apoptosis and Calcium Ion Concentration

    Xiang‑Dong Xu1, Xiang‑Qing Xu2★, Hong Yun Wu2, Zhi Jun Li3★ Corresponding author

    1. 1Departments of Laboratory, Hospital of Shandong University of Traditional Chinese Medicine, China.
    2. 2Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, China.
    3. 3The First Clinical Medical School, Shandong University of Traditional Chinese Medicine, Jinan, China.

    CORRESPONDENCE

    Xiang‑Qing Xu

    Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, China.

    xqx123doc@163.com

    Received: 31-05-2018; Revised: 28-06-2018.

    Volume 15, Issue 61 · pp. 298–303 · PUBLISHED 6 March 2019 · DOI: 10.4103/pm.pm_273_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: This study aims to investigate the effect of gardenia/ acanthopanax‑medicated serum on pheochromocytoma (PC12) cell apoptosis and calcium ion concentration, and explore the antidepression mechanism of gardenia/acanthopanax. Materials and Methods: In this study, PC12 cells were treated with high concentrations of corticosterone to simulate the effect of high level glucocorticoids in neurons in patients with depression. Next, the apoptosis rate of cells, apoptosis‑related proteins, and relative intracellular calcium concentration were detected using flow cytometry. Results: Compared with the blank group, the apoptosis rates, pro‑apoptotic proteins, and calcium ion concentrations were significantly increased, and the anti‑apoptotic proteins decreased in the control groups, and the results were reversed by different drug‑medicated serum. Compared with the control groups, the differences in gardenia and fluoxetine groups were statistically significant (P < 0.01) and were more significant than acanthopanax group (P < 0.05); but the differences between the gardenia and fluoxetine groups were not statistically significant (P > 0.05). Conclusion: The results of this study suggest that gardenia/acanthopanax‑medicated serum can obviously improve the survival rate of neurons under high concentrations of glucocorticoids, downregulate the apoptosis‑related proteins, and reduce the intracellular calcium concentration, and gardenia had better effects than acanthopanax.

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      Xu, X., Xu, X., Wu, H. Y., & Li, Z. J. (2019). Effect of Gardenia/Acanthopanax‑Medicated Serum on High Level Corticosterone‑induced Pheochromocytoma Cell Apoptosis and Calcium Ion Concentration. Pharmacognosy Magazine, 15(61), 298–303. https://doi.org/10.4103/pm.pm_273_18