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    Anti‑diabetic Activities of Dactylorhiza hatagirea Leaf Extract in 3T3‑L1 Cell Line Model

    Murad Alsawalha1, Abeer Mohammed Al-Subaei2, Reem Yousuf Al-Jindan3, Srinivasa Rao Bolla4, Dwaipayan Sen5, Janardhana Papayya Balakrishna5, Padma Kanchi Ravi6, Shiva Shankar Reddy Gollapalli7, Vishnu Priya Veeraraghavan8, Aruthra Arumugam Pillai7, Joel Palpath Joseph7, Mohammed Salahuddin9, Surapaneni Krishna Mohan10★★ Corresponding author

    1. 1Department of Chemical and Process Engineering Technology, Jubail Industrial College (JIC).
    2. 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Departments of.
    3. 3Microbiology and.
    4. 4Anatomy, College of Medicine.
    5. 5Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), VIT University, Vellore.
    6. 6Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Padmvathi Nagar, Tirupati, Andhra Pradesh, College (JIC).
    7. 7Deprtment of Biotechnology, Stellixir Biotech Private Ltd., Peenya 2nd Stage Industrial Area, Bangalore, Karnataka.
    8. 8Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Velappanchavadi, Chennai, Tamil Nadu, India.
    9. 9Animal House Department, Institute of Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia.
    10. 10Department of Medical Biochemistry, College of Applied Medical Sciences in Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, Deffi, Jubail Industrial City, Al Jubail.

    CORRESPONDENCE

    Surapaneni Krishna Mohan

    Department of Medical Biochemistry, College of Applied Medical Sciences in Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, Deffi, Jubail Industrial City, Al Jubail.

    skmohan@iau.edu.sa

    Received: 12-01-2019; Revised: 25-02-2019.

    Volume 15, Issue 64s · pp. S212–S217 · PUBLISHED 26 August 2019 · DOI: 10.4103/pm.pm_8_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Diabetes is an endocrine disorder that results in altered carbohydrate, protein, and lipid metabolism. Several synthetic drugs used to treat diabetes have adverse effects on prolonged usage. This has given the impetus to the search for alternative medicines with no or less side effects. The plants of Orchidaceae family have displayed antimicrobial, anti‑inflammatory, antioxidant, anticancer, and antidiabetic activity. However, their antidiabetic properties are yet to be explored. Materials and Methods: The in vitro antidiabetic properties of Dactylorhiza hatagirea leaf extract were studied by biochemical assays such as α‑amylase and α‑glucosidase inhibition assays and in vitro cellular assays such as glucose uptake assay and glucose transporter type 4 (GLUT4) expression studies in 3T3‑L1 cell line. Results: The methanolic extract of D. hatagirea, at varying concentrations (25 μg‑400 μg/mL), did not exhibit cytotoxicity against 3T3-L1 cell line after 24 h of incubation. Methanolic extract of D. hatagirea leaves showed significant inhibition of α‑Amylase and α‑Glucosidase enzymes. After 24 h of exposure of 3T3-L1 cells to 100 µg/mL of D. hatagirea leaf extract and 100 µM of metformin, the relative expression rates of GLUT4 receptor were elevated when compared with untreated cells. The results also revealed that the amount of 2‑(N‑(7‑Nitrobenz‑2‑oxa‑1,3‑diazol‑4‑yl) Amino)‑2‑Deoxyglucose taken up by 3T3-L1 cells treated with D. hatagirea leaf extract and metformin is higher than that of untreated cells. Conclusion: Our results suggest that methanolic extract of D. hatagirea leaves has potential antidiabetic activity and could be a plausible resource for antidiabetic agents.

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      Alsawalha, M., Al-Subaei, A. M., Al-Jindan, R. Y., Bolla, S. R., Sen, D., Balakrishna, J. P., Ravi, P. K., Gollapalli, S. S. R., Veeraraghavan, V. P., Pillai, A. A., Joseph, J. P., Salahuddin, M., & Mohan, S. K. (2019). Anti‑diabetic Activities of Dactylorhiza hatagirea Leaf Extract in 3T3‑L1 Cell Line Model. Pharmacognosy Magazine, 15(64s), S212–S217. https://doi.org/10.4103/pm.pm_8_19