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    Article

    Osteogenic Activity of Resveratrol in Human Fetal Osteoblast Cells

    Aarti Abhishek Shah1, Karthik Gourishetti1, Yogendra Nayak1★★ Corresponding author

    1. 1Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.

    CORRESPONDENCE

    Yogendra Nayak

    Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.

    yogendra.nayak@manipal.edu

    Received: 03-12-2018; Revised: 22-01-2019.

    Volume 15, Issue 64s · pp. S250–S255 · PUBLISHED 26 August 2019 · DOI: 10.4103/pm.pm_619_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The main objective of the study was to evaluate RSV on the proliferation and differentiation of immortalized human fetal osteoblastic cells 1.19 (hFOB). Materials and Methods: The osteoblastic cell proliferation and differentiation potentials of RSV were tested by cell viability assay, alkaline phosphatase (ALP) activity, total protein content, and alizarin staining for the mineralization assays. Results: The cell viability assay indicated that RSV was found to be safe at a wider concentration range and the EC50 was 72.05 µM. The therapeutic concentrations as 500 nM and 1 µM were selected for the further assays. RSV at 500‑nM and 1‑µM concentrations treatment on immortalized human fetal osteoblastic cells 1.19 (hFOB) did not show a significant effect on ALP activity. The total cellular proteins in hFOB increased in a dose‑dependent manner on RSV treatment (P ≤ 0.05). The significant staining and the color intensity of the calcium crystals by Alizarin staining assay indicate a stimulatory effect on the mineralization phase of bone formation. Conclusion: In mature osteoblasts, ALP activity is expressed in early stage, whereas mineralized nodules are formed in the late stage of differentiation. Therefore, the present study suggests that RSV stimulates the process of bone formation through activation of late differentiation phase and may have positive effects on osteoblastic differentiation potential.

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      Shah, A. A., Gourishetti, K., & Nayak, Y. (2019). Osteogenic Activity of Resveratrol in Human Fetal Osteoblast Cells. Pharmacognosy Magazine, 15(64s), S250–S255. https://doi.org/10.4103/pm.pm_619_18