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    Article

    Comparative Study of Ultra‑High‑Performance Supercritical Fluid Chromatography and Ultra‑High‑Performance Liquid Chromatography to Simultaneous Determination of Ten Components in Radix hedysari

    Bo Wang2,3, Xiao‑Hua Liu2, Zhi‑Yuan Xue2, Xiu‑Yan Yang2, Yao‑Yao Fang2, Shi‑Lan Feng2★★ Corresponding author

    1. 1Institute of Drug Analysis, School of Pharmacy, Lanzhou University, China.
    2. 2Lanzhou Customs, Food Safety Testing Laboratory, P.R, China.

    CORRESPONDENCE

    Shi‑Lan Feng

    Lanzhou Customs, Food Safety Testing Laboratory, P.R, China.

    fengshl@lzu.edu.cn

    Received: 31-05-2019; Revised: 27-06-2019.

    Volume 16, Issue 67 · pp. 99–110 · PUBLISHED 11 February 2020 · DOI: 10.4103/pm.pm_241_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: This study intends to establish a high‑throughput method for simultaneous determination of ten compounds in RH by UHPSFC and to develop a standardized method to comprehensively evaluate the quality of RH samples. In addition, this study was based on UHPLC and UHPSFC systems to understand and compare the effects of different separation parameters on ten target compounds in RH. A high‑throughput and reliable solid‑phase extraction method for simultaneous detection of ten target compounds in RH was established and validated. Results: The optimal analysis method was achieved using HSS C18 SB column, gradient elution, flow rate of 1.5 mL/min, diode array detection at 270 nm, and back pressure at 12.41 Mpa. The separation method was validated sufficiently by examining the precision, recoveries, linearity, range, limit of detection, and limit of quantification and was successfully applied to quantify the 25 RH samples. In addition, the comparative study also demonstrated the complementary nature of the two separation modes. Conclusion: This is the first study that ten compounds in RH were separated and analyzed simultaneously with UHPSFC‑diode array detector. In addition, under the separation conditions of UHPSFC and UHPLC, the comparison of chromatographic parameters shows that a combination of these two separation techniques efficiently separates the ten target compounds in RH. The results obtained in this study are useful for future TCM quality control and high‑throughput analysis.

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      Wang, B., Liu, X., Xue, Z., Yang, X., Fang, Y., & Feng, S. (2020). Comparative Study of Ultra‑High‑Performance Supercritical Fluid Chromatography and Ultra‑High‑Performance Liquid Chromatography to Simultaneous Determination of Ten Components in Radix hedysari. Pharmacognosy Magazine, 16(67), 99–110. https://doi.org/10.4103/pm.pm_241_19