Response Surface Optimization of Prodigiosin Production by Mutagen‑Treated Serratia marcescens in Different Growth Media
Sumaira Gul1, Muhammad Aslam Shad2, Rubina Arshad3, Haq Nawaz2★, Aleema Ali2, Asma Altaf2, Tehreem Gul2, Wafa Iqbal2★ Corresponding author
- 1Departments of Chemistry, University, Multan 60800, Pakistan.
- 2Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
- 3Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan.
CORRESPONDENCE
Haq Nawaz
Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
Received: 25-09-2019; Revised: 13-11-2019.
Volume 16, Issue 68S · pp. S99–107 · PUBLISHED 31 March 2020 · DOI: 10.4103/pm.pm_430_19
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ABSTRACT
Objectives: The study was planned to optimize the effect of ultravoilet (UV) light and acridine orange (AO), as mutagens and incubation time on prodigiosin production by S. marcescens in nutrient agar broth and peptone glycerol broth. Materials and Methods: The optimization was done by constructuing a quadratic polynomial response surface model using a bifactorial central composite design. The S. marcescens starian was treated with UV light (0, 30, 60, 90, and 120 s) and various concentrations of AO (0, 50, 100, 150, and 200 µg/mL) and incubated in the selected growth media for different times (0, 15, 30, 45, and 60 h). The prodigiosin production was measured in terms of change in pH and optical density (OD) of the ethanolic extracts of culture media. Results: The prodigiosin production was found to be a significant linear and quadratic function of incubation time in both of the culture media. However, a statistically non-significant increased prodigiosin production, in terms of pH and OD of the extracts, was observed under the influence of both mutagens. The optimum values of incubation time to achieve optimal production of prodigiosin from UV light‑exposed and AO‑treated cultures were found to be in the range of 37–60 h, respectively. Conclusion: The pigment production was increased significantly with an increase in the incubation time. The study provides the optimum conditions for the production of prodigiosin in research and commercial field.
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Gul, S., Shad, M. A., Arshad, R., Nawaz, H., Ali, A., Altaf, A., Gul, T., & Iqbal, W. (2020). Response Surface Optimization of Prodigiosin Production by Mutagen‑Treated Serratia marcescens in Different Growth Media. Pharmacognosy Magazine, 16(68S), S99–107. https://doi.org/10.4103/pm.pm_430_19