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    Article

    Simultaneous Determination of two Diterpenoids, Continentalic Acid and Kaurenoic Acid, in the Water extract of Aralia continentalis and their Wound‑Healing Activity

    Guijae Yoo1, Taek Hwan Lee2, Jae Hyuk Lee3, Kyo Bin Kang4, Heejung Yang5, Yong Kon Park1, Sun Yeou Kim3, Seung Hyun Kim2★★ Corresponding author

    1. 1Korea Food Research Institute, Jeollabuk‑do, 1 College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University.
    2. 2College of Pharmacy, Gachon University, Incheon.
    3. 3College of Pharmacy, Sookmyung Women’s University, Seoul.
    4. 4College of Pharmacy, Kangwon National University, Chuncheon, Korea.

    CORRESPONDENCE

    Seung Hyun Kim

    College of Pharmacy, Gachon University, Incheon.

    kimsh11@yonsei.ac.kr

    Received: 10-08-2020; Revised: 10-09-2020; Accepted: 01-12-2020.

    Volume 16, Issue 72 · pp. 745–749 · PUBLISHED 16 February 2021 · DOI: 10.4103/pm.pm_344_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: Simultaneous determination of two diterpenoids; continentalic acid (CA) and kaurenoic acid, in the water extract of A. continentalis using the high‑performance liquid chromatography (HPLC) was established for quality control, as well as evaluation of their wound‑healing activity. Materials and Methods: The separation was conducted on YMC hydrosphere C18 column with isocratic elution of 65% acetonitrile. Furthermore, the isolated diterpenoids were screened for their wound‑healing activity using keratinocytes (HaCaT cells). Results: The calibration curves were linear over the established range with R2 > 0.9999. The intraday and interday RSDs for each compound were 0.13%–0.89% and 0.14%–0.73%, respectively. The limits of detection and limits of quantification for the two tested diterpenoids were 0.0912, 0.0018 μg/μL, and 0.2764, 0.0056 μg/μL, respectively. In addition, the CA‑treated groups showed accelerated wound closure compared to the control group. Conclusion: The HPLC method could be accomplished to the quality control and stable experiment for the preparations consisted of two diterpenoids. Compared to the control group, the CA‑treated group showed that wound closure was accelerated, indicating its potential in promoting migration of skin cells which is the most important step of wound closure.

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      Yoo, G., Lee, T. H., Lee, J. H., Kang, K. B., Yang, H., Park, Y. K., Kim, S. Y., & Kim, S. H. (2021). Simultaneous Determination of two Diterpenoids, Continentalic Acid and Kaurenoic Acid, in the Water extract of Aralia continentalis and their Wound‑Healing Activity. Pharmacognosy Magazine, 16(72), 745–749. https://doi.org/10.4103/pm.pm_344_20