Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Anti-inflammatory and antioxidant activities of Cissus quadrangularis stem extract

    Manmohit Kalia1, Devendra Singh1, Deepmala Sharma2, Shahid Suhail Narvi3, Vishnu Agarwal1★★ Corresponding author

    1. 1Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, Uttar Pradesh, India.
    2. 2Department of Mathematics, National Institute of Technology, Raipur, Chhattisgarh, India.
    3. 3Department of Chemistry, Motilal Nehru National Institute of Technology, Allahabad, Uttar Pradesh, India.

    CORRESPONDENCE

    Vishnu Agarwal

    Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, Uttar Pradesh, India.

    vishnua@mnnit.ac.in

    Received: 03-08-2020; Revised: 09-09-2020; Accepted: 01-12-2020.

    Volume 16, Issue 72 · pp. 797–802 · PUBLISHED 16 February 2021 · DOI: 10.4103/pm.pm_315_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Aim: This study was carried out with the objective to attenuate quorum sensing (QS)‑controlled virulence factors in P. aeruginosa and its biofilm. Methods: In the present study, acetone and hexane extracts of S. alexandriana mill were tested at sub‑minimum inhibitory concentration concentrations for their anti‑QS activity using bioreporter strain the Chromobacterium violaceum CV026. The effect of S. alexandriana Mill on different virulence phenotypes such as pyocyanin and protease production was investigated. Finally, the effect of the extract on P. aeruginosa, biofilm formation was also determined using the spectrophotometry‑based method and microscopic analysis. Results: The extracts were tested for their activities against QS‑controlled virulence factors of P. aeruginosa at 600 µg/ml and 800 µg/ml acetone and hexane extract concentration, respectively. Results showed that both the extracts have an inhibitory effect on different virulence factors of P. aeruginosa, including pyocyanin, protease production, and biofilm formation, without affecting the growth. The P. aeruginosa biofilm was found to be decreased by 75% and 62% in acetone and hexane extract presence. Conclusion: The results revealed that both acetone and hexane extracts showed anti‑biofilm and anti‑QS activity. It depicted the potential of S. alexandriana Mill against P. aeruginosa infection, its biofilm, and QS.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Kalia, M., Singh, D., Sharma, D., Narvi, S. S., & Agarwal, V. (2021). Anti-inflammatory and antioxidant activities of Cissus quadrangularis stem extract. Pharmacognosy Magazine, 16(72), 797–802. https://doi.org/10.4103/pm.pm_315_20