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    Ethanolic Extract of Capparis decidua Fruit Ameliorates Methotrexate‑Induced Hepatotoxicity by Suppressing Oxidative Stress and Inflammation by Modulating Nuclear Factor‑Kappa B Signaling Pathway

    Preetha Santhakumar1★, Anitha Roy2, M. Karthik Ganesh3, J. Selvaraj4, Lavanya Prathap3, Yuvaraj Babu K3★ Corresponding author

    1. 1Departments of Physiology, College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.
    2. 2Pharmacology, India.
    3. 3Anatomy and, India.
    4. 4Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.

    CORRESPONDENCE

    Preetha Santhakumar

    Departments of Physiology, College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.

    drpreeth.homeo@gmail.com

    Received: 06-09-2020; Revised: 08-02-2021; Accepted: 22-04-2021.

    Volume 17, Issue 6S · pp. S143–S150 · PUBLISHED 15 September 2021 · DOI: 10.4103/pm.pm_402_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study investigated the protective effect of C. decidua against methotrexate‑induced hepatotoxicity, focusing on its ability to attenuate oxidative stress and inflammatory nuclear factor‑kappa B (NF‑κB) signaling pathway which have not been studied earlier. Materials and Methods: Thirty‑six female Wistar rats were randomly divided into six experimental groups. Rats were treated with C. decidua orally with doses of 250 mg/kg and 500 mg/kg for 14 consecutive days following a single dose of MTX injection (20 mg/kg, i. p). Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were assessed. Malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) were assessed in hepatic tissue. mRNA expression of, NF‑κB, tumor necrosis factor‑alpha (TNF α), interleukin‑1beta (IL‑1 β), and IL 6 was carried using real‑time polymerase chain reaction in hepatic tissue. Results: Results showed that methotrexate administration significantly increased AST, ALT, and ALP levels. It has also increased MDA levels indicating lipid peroxidation and decreased antioxidants levels of SOD and CAT levels. Treatment with C. decidua significantly diminished the hepatotoxic effects of methotrexate which was seen in a significant decrease in AST, ALT, ALP, MDA levels, significant increase in SOD and CAT levels. Methotrexate provoked hepatic NF‑κB phosphorylation and increased mRNA abundance of TNF‑α, IL‑1 β, and IL‑6 expression. Conclusion: These findings suggest that C. decidua prevents methotrexate hepatotoxicity through suppression of NF‑κB signaling pathway, attenuating oxidative damage, inflammation, and cell death.

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      Santhakumar, P., Roy, A., Ganesh, M. K., Selvaraj, J., Prathap, L., & K, Y. B. (2021). Ethanolic Extract of Capparis decidua Fruit Ameliorates Methotrexate‑Induced Hepatotoxicity by Suppressing Oxidative Stress and Inflammation by Modulating Nuclear Factor‑Kappa B Signaling Pathway. Pharmacognosy Magazine, 17(6S), S143–S150. https://doi.org/10.4103/pm.pm_402_20