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    Article

    Celastrus paniculatus Seed Oil Ameliorates Oxidative Stress in Lipopolysaccharide‑Induced Respiratory Inflammation in Mice

    William R. Surin1★, Eveline J. Sharon1, Archana Kullu2, Santhosh Kacham1, Sandya S2★ Corresponding author

    1. 1Departments of Microbiology and Cell Biology, Institute of Science, Bengaluru, Karnataka, India.
    2. 2Inorganic and Physical Chemistry, Indian Institute of Science, Bengaluru, Karnataka, India.

    CORRESPONDENCE

    William R. Surin

    Departments of Microbiology and Cell Biology, Institute of Science, Bengaluru, Karnataka, India.

    wrsurin@iisc.ac.in

    Received: 14-12-2021; Revised: 10-01-2022; Accepted: 04-04-2022.

    Volume 18, Issue 78 · pp. 400–405 · PUBLISHED 7 July 2022 · DOI: 10.4103/pm.pm_575_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Celastrus paniculatus have been reported to possess various medicinal properties. However, very little is known about itseffect on respiratory inflammation. Objectives: We evaluated Celastrus paniculatus seed oil (CP oil) on lipopolysaccharide (LPS)‑induced mice model of respiratory inflammation. Materials and Methods: Both short‑term and long‑term studies were carried out. In short‑term study, C57BL/6 mice were exposed to LPS (10 mg/kg) by intranasal route for one week. CP oil (1 g/kg) was given orally on the 1st, 3rd and 5th day. All the mice were sacrificed on the 7th day. In long‑term study, C57BL/6 mice were exposed to LPS (10 mg/kg) by the intranasal route for one month. CP oil (1 g/kg) or theophylline (1 mg/kg) was administered twice a week for one month. At the end of the treatment, Bronchoalveolar lavage (BAL) fluid was collected and labelled with CD3e FITC, Ly‑6G (Gr‑1) PE‑Cy‑7, Allergin‑1 eFlour® 660, CD14 APC and 2’,7’‑dichlorofluorescein diacetate (20 µM) and analyzed by flow cytometer (BD FACSCanto II) for cellular infiltration and reactive oxygen species (ROS) generation. There was a significant reduction in neutrophil infiltration following CP oil treatment for one month ( 68±6% vs 40±7%, P < 0.05). Furthermore, there was a significant decrease in ROS generation following CP oil treatment for one week (5873 ± 1133 vs 2581 ± 1359; P < 0.05) and one month (20618 ± 1854 vs 5850 ± 1006; P < 0.05). Further, theophylline treatment for one month reduced the ROS generation significantly (20618 ± 1854 vs 5286 ± 2413; P < 0.05). Conclusion: Celastrus paniculatus seed oil seems to reduce oxidative stress by inhibiting the generation of reactive oxygen species in LPS‑induced lung inflammation.

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      Surin, W. R., Sharon, E. J., Kullu, A., Kacham, S., & S, S. (2022). Celastrus paniculatus Seed Oil Ameliorates Oxidative Stress in Lipopolysaccharide‑Induced Respiratory Inflammation in Mice. Pharmacognosy Magazine, 18(78), 400–405. https://doi.org/10.4103/pm.pm_575_21