Network Pharmacology Analysis and Experimental Validation of the Antidepression Mechanism of Lactuside B

ABSTRACT

Objectives: This study was the first to study the pharmacological effects and mechanisms of LB on depression. Materials and Methods: Network pharmacology method was applied to screen the candidate targets and signaling pathways. Furthermore, molecular docking, cell test, and animal experiments were used to confirm the antidepression mechanisms. Results: The network pharmacology results showed 29 targets and 40 signaling pathways of LB on depression. Molecular docking showed that there was a strong binding effect between LB and each target (cAMP response element binding protein B [CREB], Ras, Raf, and ERK1/2). Cell tests indicated that the expressions of Ras and CREB in astrocyte cells (0.05–0.20 g/L of LB) exhibited significant differences (P < 0.05) compared with the model group, respectively. In animal tests, the expressions of Ras, ERK1/2, and Raf (12.5–50.0 µg/10 g of LB) showed a significant difference compared with mice in the model group (P < 0.05). Conclusion: These results indicated that the antidepression mechanism of LB was mainly associated with Ras signaling pathway, and the cAMP signaling pathway and PI3K‑Akt signaling pathway may play an essential role.

KEYWORDS

• Depression
• lactuside B
• molecular mechanism
• network pharmacology
• target

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• DOI10.4103/pm.pm_79_22Copy

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