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    Article

    Integrating Background Subtraction and Metabolomics Technology to Study the Difference of Serum Chemical Profile Before and After Compatibility of Radix Bupleuri and Radix Paeoniae Alba

    Congcong Chen1★, Ruihong Hou1★, Qicai Yin2,3, Junshen Tian2,3, Xiaoxia Gao2,3, Xuemei Qin2,3, Guanhua Du2,5, Yuzhi Zhou2,3★★ Corresponding author

    1. 1Modern Chinese Medicine Research Center, Modern Research Center for Traditional Chinese Medicine, the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, No. 92, Wucheng Road, Taiyuan, Shanxi, China.
    2. 2Shanxi Provincial Key Laboratory of Research and Utilization of Chinese Medicine Active Substances, Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Shanxi University, No. 92, Wucheng Road, Taiyuan, Shanxi, China.
    3. 3Department of Rheumatology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Shanxi Bethune Hospital Affiliated to Shanxi Medical University, Taiyuan, Shanxi, China.
    4. 4Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P. R, China.

    CORRESPONDENCE

    Congcong Chen

    Modern Chinese Medicine Research Center, Modern Research Center for Traditional Chinese Medicine, the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, No. 92, Wucheng Road, Taiyuan, Shanxi, China.

    zhouyuzhi@sxu.edu.cn

    Received: 30-03-2022; Revised: 20-04-2022; Accepted: 01-08-2022.

    Volume 18, Issue 80 · pp. 1160–1169 · PUBLISHED 23 November 2022 · DOI: 10.4103/pm.pm_139_22

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: Here, an effective strategy has been developed for screening differences of constituents in vivo before and after compatibility by integrating background subtraction and metabolomics technology. Materials and Methods: First, to obtain the chemical composition from the CH in vivo before compatibility, the Liquid chromatography/Mass spectrometry (LC/MS) data of serum samples in the control group were subtracted from serum samples in the CH group based on the background subtraction method. Moreover, the chemical composition from the CH in vivo after compatibility was obtained by subtracting the LC/MS data of serum samples in the BS group from serum samples in the CH‑BS compatibility group. Finally, the difference in the chemical composition from the CH in vivo before and after compatibility was screened and analyzed by metabolomics. Meanwhile, the difference in chemical composition from the BS in vivo before and after the compatibility was found according to the same thought with BS. Results: The results showed that three prototype components were significantly decreased and seven metabolites were significantly increased in CH after compatibility. And eight prototype components and six metabolites were significantly increased in BS after compatibility. Conclusion: An effective strategy has been developed for screening differences of constituents in vivo before and after compatibility by combining background subtraction and metabolomics.

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      Chen, C., Hou, R., Yin, Q., Tian, J., Gao, X., Qin, X., Du, G., & Zhou, Y. (2022). Integrating Background Subtraction and Metabolomics Technology to Study the Difference of Serum Chemical Profile Before and After Compatibility of Radix Bupleuri and Radix Paeoniae Alba. Pharmacognosy Magazine, 18(80), 1160–1169. https://doi.org/10.4103/pm.pm_139_22