Apoptosis‑Mediated Anticancer Activity of Geraniol Inhibits NF‑κB, MAPK, and JAK‑STAT‑3 Signaling Pathways in Human Thyroid Cancer Cells
Hongli Yang1★, Yonggang Zhang1★, Jianying Cui2, Zhi Hao1★★ Corresponding author
- 1Department of Head and Neck Surgery, Hospital of Hebei University, Baoding, China.
- 2Administration Office of Medical Record, Affiliated Hospital of Hebei University, Baoding, China.
CORRESPONDENCE
Hongli Yang
Department of Head and Neck Surgery, Hospital of Hebei University, Baoding, China.
Received: 20-05-2021; Revised: 16-11-2021; Accepted: 01-08-2022.
Volume 18, Issue 80 · pp. 1183–1189 · PUBLISHED 23 November 2022 · DOI: 10.4103/pm.pm_223_21
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ABSTRACT
Aim: Thyroid cancer accounts for 3.8% of the total endocrine cancer and is the fifth most diagnosed cancer among Indian women. Geraniol possesses pharmacological properties, including anti‑inflammatory, anti‑cancer, and neuroprotective properties. The anti‑cancer activity of geraniol on TPC‑1 cells was assessed in this study. Materials and Methods: The anticancer activity of geraniol in TPC‑1 cells was evaluated by MTT test, ROS, DAPI, PI, and AO/EB staining. mRNA and expression were assessed by qPCR and western blot. Results: The geraniol has shown cytotoxic effects toward TPC‑1 cells and showed an IC50 value of 25 µM, which decreases cell viability and inhibited cell proliferation. It also increased the accumulation of intracellular ROS, which induces apoptosis in the TPC‑1 cells due to the dissipation of membrane potential and disruption of mitochondria. There is a substantial reduction in the mRNA level expressions of Bcl‑2, Cyclin D1, c‑Myc, COX‑2, TNF‑α, NF‑κB, IL‑6, and surviving. It also upregulated the Bax and caspase‑3 mRNA expression suggesting cell death‑mediated apoptosis. Conclusion: We report that geraniol effectively inhibited the JAK‑2, STAT‑3, and ERK signaling pathways depicting geraniol as a potential therapeutic molecule for thyroid cancer treatment.
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Yang, H., Zhang, Y., Cui, J., & Hao, Z. (2022). Apoptosis‑Mediated Anticancer Activity of Geraniol Inhibits NF‑κB, MAPK, and JAK‑STAT‑3 Signaling Pathways in Human Thyroid Cancer Cells. Pharmacognosy Magazine, 18(80), 1183–1189. https://doi.org/10.4103/pm.pm_223_21