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    Pharmacognosy MagazineNOW IN
    Article

    Neuroprotective Effects of Bornyl Acetate against Okadaic Acid–Induced Cytotoxicity in PC12 Cells Via Beclin‑1‑Dependent Autophagy Pathway

    Liping Huang2,3, Xiaoqin Zhong4, Zhongliu Zhou2,3, NanBu Wang4, MinZhen Deng5,6,7★★ Corresponding author

    1. 1School of Chemistry and Chemical Engineering, Western Guangdong Characteristic Biomedical Engineering Technology Research Center, China.
    2. 2Mangrove Institute, Lingnan Normal University, Zhanjiang 524048, China.
    3. 3The First Clinical College of Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou, Guangdong 510405, China.
    4. 4Department of Neurology, Guangdong Provincial Hospital of Chinese Medicine, China.
    5. 5Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, China.
    6. 6Postdoctoral Research Station of Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong 510120, P.R, China.

    CORRESPONDENCE

    MinZhen Deng

    Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, China.

    dengmz1@126.com

    Received: 08-12-2021; Revised: 16-01-2022; Accepted: 27-04-2022.

    Volume 18, Issue 80 · pp. 962–968 · PUBLISHED 23 November 2022 · DOI: 10.4103/pm.pm_565_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The purpose of our experiment was to elucidate the mechanism of treatment of AD. Materials and Methods: To explore how BA has therapeutic effects on AD, a model of OA‑induced PC12 cells was established. The OA modelling induction and BA treatment in cells were evaluated by LDH and CCK‑8 methods; ELISA assay was used to detect the tau hyperphosphorylation (p‑tau), Aβ42 and β‑secretase levels; The expression of p‑Akt and p‑mTOR were detected by western blot analysis; and immunohistochemical, immunofluorescence methods and western blot analysis were used to detect Beclin‑1 expression. Autophagosomes in each group were observed by transmission electron microscopy (TEM). 175 nM OA for 48 hr was applied to OA modelling induction. Results: Compared to the control group, the OA‑induced AD model cells displayed higher levels of p‑tau, Aβ42 and β‑secretase (P < 0.01), suggesting that the AD model was successfully established. Compared to the OA model group alone, p‑tau, Aβ42 and β‑secretase levels and autophagy promoter Beclin‑1 expression decreased significantly in the BA group (P < 0.05), whereas p‑Akt and p‑mTOR increased (P < 0.01). Conclusion: BA exhibited a neuroprotection effect against OA‑induced cellular injury in the AD model by suppressing the Beclin‑1‑dependent autophagy pathway, indicating that BA might be an appealing potential strategy to treat AD.

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      Huang, L., Zhong, X., Zhou, Z., Wang, N., & Deng, M. (2022). Neuroprotective Effects of Bornyl Acetate against Okadaic Acid–Induced Cytotoxicity in PC12 Cells Via Beclin‑1‑Dependent Autophagy Pathway. Pharmacognosy Magazine, 18(80), 962–968. https://doi.org/10.4103/pm.pm_565_21