Inhibitory Activities of Extracts from Chinese Medicinal Herbs on α-Amylase, α-Glucosidase, and Pancreatic Lipase
Xiang Liu1, Yuhao Nie1, Rui Chen1, Xiaoying Zhang1, Lijuan Yue2, Chen Chen1★★ Corresponding author
- 1Chinese-German Joint Laboratory for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China.
- 2Department of Oncology, Hanzhong Central Hospital, Hanzhong, Shaanxi, China.
CORRESPONDENCE
Chen Chen
Chinese-German Joint Laboratory for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China.
Volume 19, Issue 3 · pp. 530–538 · PUBLISHED 2023 · DOI: 10.1177/09731296231168739
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ABSTRACT
Background: Metabolic syndrome is an assortment of conditions that often happens together and upsurge your risk of diabetes, stroke, and heart disease. One strategy for the prophylaxis and treatment of diabetes and obesity is to inhibit the enzymes activities, which include α-glucosidase, α-amylase, and pancreatic lipase. Objectives: To screen the effect of 16 Chinese medicinal herbs on inhibition of α-glucosidase, α-amylase, and pancreatic lipase. Materials and Methods: The water extraction of 16 traditional Chinese herbal medicines was used to estimate activity in vitro from different families against α-glucosidase, α-amylase, and pancreatic lipase by using spectrophotometry with p-nitrophenyl-α-D-glucopyranoside (PNPG), starch derivatives, and 4-methylumbelliferone (4-MUO), respectively, as a substrate. Results: The results showed that the water extraction yield of the 16 rhizomes ranged from 0.2% to 4.0%. Among the tested extracts, the extract from Gastrodia elata exhibited the strongest effect on α-amylase (The half-maximal inhibitory concentration (IC50)) = 4.10 mg/mL). The extracts from Pinellia ternate and Arisaema heterophyllum exhibited noteworthy effects on α-glucosidase (IC50 = 29.21 and 41.13 µg/mL, respectively), and Corydalis turtschaninovii possessed the highest effect on pancreatic lipase (IC50 = 4.11 µg/mL) and a stronger effect than orlistat (IC50 = 4.52 µg/mL). Conclusion: This study provides a basis for screenings and in-depth studies of anti-obesity and anti-diabetes drugs. Further isolation, identification, and characterization of active compounds should be carried out in the future.
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Liu, X., Nie, Y., Chen, R., Zhang, X., Yue, L., & Chen, C. (2023). Inhibitory Activities of Extracts from Chinese Medicinal Herbs on α-Amylase, α-Glucosidase, and Pancreatic Lipase. Pharmacognosy Magazine, 19(3), 530–538. https://doi.org/10.1177/09731296231168739