Phytochemical and Anti-inflammatory Activities of Oxytropis aciphylla
Hao Qian1, Jiaqiong Liu1, Xianqi Meng1, Xiaoqin Wang1★★ Corresponding author
- 1Department of Pharmacy, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
CORRESPONDENCE
Xiaoqin Wang
Department of Pharmacy, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Received: 29-10-2023; Accepted: 06-02-2024.
Volume 20, Issue 3 · pp. 945–955 · PUBLISHED 2024 · DOI: 10.1177/09731296241240236
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ABSTRACT
Background: The objective of this research is to explore the phytochemical constituents and the potential anti-inflammatory properties of the herb Oxytropis aciphylla. Materials and Methods: From the ethyl acetate and n-butanol fractions derived from the 70% ethanol extract of O. aciphylla, a total of 17 distinct chemical entities were extracted and subsequently identified. Utilizing network pharmacology, the study predicted the possible anti-inflammatory targets and mechanisms of action of O. aciphylla. The study assessed the antiinflammatory impact of O. aciphylla extract on RAW 264.7 (a murine macrophage cell line) cells induced with lipopolysaccharide (LPS) by quantifying the concentrations of nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Results: The isolated compounds included 12 flavonoids, two triterpenoids, one fatty acid, and two sterols. These specifically comprise apigenin, luteolin, kaempferol, isorhamnetin, naringenin, eriodictyol, dihydrokaempferol, butein, 7,4’-homoisoflavanone, apigenin-7-O-β-D-glucopyranoside, isorhamnetin-3-O-β-D-gentiobioside, rutin, azukisapogenol, 3-O-[β-D-glucopyranosyl(1→2)-β-D-glucuronopyranosyl] azukisapogenol methyl ester, tetracosanoic acid, daucosterol, and β-sitosterol. Network pharmacology analysis suggested that O. aciphylla may exert its anti-inflammatory effects through multiple components, targets, and pathways, such as kaempferol, apigenin, luteolin, AKT1, SRC, TNF, PTGS2, EGFR, MAPK, PI3K-Akt, VEGF, HIF-1, and TNF signaling pathways. The extract of O. aciphylla was found to markedly reduce the levels of NO, TNF-α, and IL-6 in RAW 264.7 cells that were stimulated with LPS, demonstrating a correlation with the dosage administered. Conclusion: The investigation uncovered, for the first time, the phytochemical constituents and the mechanisms underlying the anti-inflammatory effects of O. aciphylla. These findings lay the groundwork for the scientific development and application of this botanical species as a natural remedy for inflammation.
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Qian, H., Liu, J., Meng, X., & Wang, X. (2024). Phytochemical and Anti-inflammatory Activities of Oxytropis aciphylla. Pharmacognosy Magazine, 20(3), 945–955. https://doi.org/10.1177/09731296241240236