Development of a simple and sensitive spectrophotometric method for the simultaneous determination of asiaticoside and wedelolactone in a polyherbal formulation.
Pallavi Rai1★, S. H. Mishra1★ Corresponding author
- 1Pharmacy Department, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India 390 001, India.
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Pallavi Rai
Pharmacy Department, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India 390 001, India.
Volume 3, Issue 9 · pp. 47–48 · PUBLISHED 2007 · DOI:
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ABSTRACT
A simple, sensitive spectroscopic method was developed for the simultaneous determination of asiaticoside and wedelolactone in mixture of Centella asiatica extract and Eclipta alba extract as well as in a polyherbal formulation containing these extracts. Asiaticoside has an absorption maxima at 278nm, the molecule obeys Beer’s law in the concentration range 10-100 µg/mL, while wedelolactone has the absorption maxima at 351nm and the linearity range is 1-10 µg/mL. The method for simultaneous estimation was based on the property of additive values of absorbance. The proposed method is reproducible and statistically validated. The average % recovery for asiaticoside is 98.63 % and for wedelolactone is 98.41 %. The developed method is an effective approach for simultaneous quantitative determination, which could be used for quality control of polyherbal preparations containing extracts of Centella asiatica and Eclipta alba.
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Rai, P., & Mishra, S. H. (2007). Development of a simple and sensitive spectrophotometric method for the simultaneous determination of asiaticoside and wedelolactone in a polyherbal formulation.. Pharmacognosy Magazine, 3(9), 47–48.