A high performance thin layer chromatography method for quantitative estimation of Diosgenin in Solanum nigrum Linn.
A. C. Suthar1★, R. M. Mulani1★ Corresponding author
- 1Botany Research Laboratory, Seth L. U. J. & Sir M. V. Collage, Andheri (East) Mumbai 400 069. India, India.
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A. C. Suthar
Suthar A. C.,Botany Research Laboratory, Seth L. U. J. & Sir M. V. Collage, Andheri (East) Mumbai 400 069. India, India.
Volume 4, Issue 14 · pp. 112–113 · PUBLISHED 2008 · DOI:
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ABSTRACT
The present paper deals with development and standardization of HPTLC method used for quantification of Diosgenin in Solanum nigrum. Solanum nigrum Linn. (Makoi) Solanaceae is known in Ayurveda for its effective treatment in cough, cold and asthma from ancient times. It contains many phyto-constituents such as Diosgenin, Solasodine & other steroidal alkaloids. As there is no analytical method for quantification of Diosgenin, which can be used for standardization of extract of Solanum nigrum, an attempt has been made to quantify Diosgenin in Solanum nigrum fresh fruit, dried crude fruit and extracts by HPTLC method. The lowest detectable limit of Diosgenin was found upto 200 nanograms and provides good resolution and separation of Diosgenin from other constituents of Solanum nigrum Linn. Further, recovery values of Diosgenin were found to be about 98%, which shows the reliability and suitability of the method. This HPTLC method was found to be reproducible, accurate and precise. The structure of isolated Diosgenin was characterized and confirmed by various advanced spectroscopic methods.
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Suthar, A. C., & Mulani, R. M. (2008). A high performance thin layer chromatography method for quantitative estimation of Diosgenin in Solanum nigrum Linn.. Pharmacognosy Magazine, 4(14), 112–113.