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    In vitro effects of extract of Senna alata (Ceasalpiniaceae) on the polyamines produced by Leukaemia cells (L1210)

    C. A. Pieme1★, V. N. Penlap1, J. Ngogang2, V. Kuete1, V. Catros3, J. Ph. Moulinoux3★ Corresponding author

    1. 1Microbiology Laboratory, Department of Biochemistry, Faculty of Science, P.o. Box 812, University of Yaounde I, Cameroon.
    2. 2Medical Biochemistry Laboratory, Faculty of Medicine and Biomedical Sciences, P.O. Box 2787, University of Yaounde I, Cameroon.
    3. 3Groupe de Recherche en Thérapeutique Anticancéreuse, Laboratoire de Biologie Cellulaire, Histologie, Embryologie, et Cytogénétique, Faculté de Médecine, Université de Rennes I, 2 avenue du Professeur Léon-Bernard, 35015 Rennes Cédex (France), France.

    CORRESPONDENCE

    C. A. Pieme

    Microbiology Laboratory, Department of Biochemistry, Faculty of Science, P.o. Box 812, University of Yaounde I, Cameroon.

    apieme@yahoo.fr

    Received: 02-05-2008; Revised: 06-11-2008; Accepted: 06-11-2008.

    Volume 5, Issue 17 · pp. 8–13 · PUBLISHED 2009 · DOI:

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    The present study reports the effects of S. alata (Ceasalpiniaceae) extract on the metabolism of polyamines resulting from the proliferation of leukaemia cells (L1210). The results established that the inhibition of cell proliferation was significantly increased with the concentration of extract from 28 to 32.80 % after 72 h. The percentage of cells viability changed significantly from 9.72 to 80 % when cells are treated with extract alone, in combination with DFMO or putrescine. The levels of the intracellular yield of putrescine, spermidine and spermine were also reduced by the extract compared to the control. The DFMO-extract complex enhanced the inhibition of the production polyamines up to 95 %. In opposite, the complex S. alata- putrescine complex stimulated significantly its biosynthesis of polyamines. A significant reduction of the level of protein after 72 h of treatment was observed. This result corroborated with the reduction of polyamines resulting from inhibition cell proliferation.

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      Pieme, C. A., Penlap, V. N., Ngogang, J., Kuete, V., Catros, V., & Moulinoux, J. P. (2009). In vitro effects of extract of Senna alata (Ceasalpiniaceae) on the polyamines produced by Leukaemia cells (L1210). Pharmacognosy Magazine, 5(17), 8–13.