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    Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton

    Wei Kun-Hua1★, Gao Shan-Lin1, Huang He-Ping1★ Corresponding author

    1. 1Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People’s Republic of, China.

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    Wei Kun-Hua

    Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, Jiangsu - 211 198, People’s Republic of, China.

    Received: 23-04-2010; Revised: 01-09-2010.

    Volume 6, Issue 24 · pp. 286–292 · PUBLISHED 20 October 2010 · DOI: 10.4103/0973-1296.71793

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Sophora flavescens Aiton is an important medicinal plant in China. Early in vitro researches of S. flavescens were focused on callus induction and cell suspension culture, only a few were concerned with in vitro multiplication. Objective: To establish and optimize the rapid propagation technology of S. flavescens and to generate and characterize polyploid plants of S. flavescens. Materials and Methods: The different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and kinetin (KT) were used to establish and screen the optimal rapid propagation technology of S. flavescens by orthogonal test; 0.2% colchicine solution was used to induce polyploid plants and the induced buds were identified by root-tip chromosome determination and stomatal apparatus observation. Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog medium (MS; 1962) supplemented with 1.4–1.6 mg/l 6-benzylaminopurine (BAP) and 0.3 mg/l indole-3-acetic acid (IAA). More than 50 lines of autotetraploid plants were obtained. The chromosome number of the autotetraploid plantlet was 2n = 4x = 36. All tetraploid plants showed typical polyploid characteristics. Conclusion: Obtained autotetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

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      Kun-Hua, W., Shan-Lin, G., & He-Ping, H. (2010). Tissue culture and generation of autotetraploid plants of Sophora flavescens Aiton. Pharmacognosy Magazine, 6(24), 286–292. https://doi.org/10.4103/0973-1296.71793