Cytotoxic and growth inhibitory effects of the methanol extract Struchium sparganophora Ktze (Asteraceae) leaves
B. A. Ayinde1★, U. Agbakwuru1★ Corresponding author
- 1Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
CORRESPONDENCE
B. A. Ayinde
Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
Received: 20-04-2010; Revised: 17-05-2010.
Volume 6, Issue 24 · pp. 293–297 · PUBLISHED 20 October 2010 · DOI: 10.4103/0973-1296.71795
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Background: Global research into medicinal plants used in treating tumor-related ailments has become imperative due to the emergence of various forms of cancer diseases. Usually consumed as a vegetable, Struchium sparganophora is indicated in traditional herbal medicine as one of the plants used in treating tumor-related ailments. Materials and Methods: This claim was examined using bench-top assay methods involving the cytotoxicity of the methanol extract of the leaves to tadpoles of Raniceps ranninus at 10, 20, 40 and 80 μg/ml. Also, the growth inhibitory effects of the extract on guinea corn radicle at 0.5, 1.0, 2 and 4 mg/ml in addition to evaluation of the phytochemical constituents of the leaves was performed. After 24 h, the crude extract and the chloroform fraction produced the highest cytotoxicity of 96.67 ± 4.71%, each at a concentration of 80 μg/ml, while the aqueous fraction produced 100% cytotoxicity at a concentration of 20 μg/ml. Results: The crude extract had an LC50 of 26 μg/ml, the chloroform fraction had 6.25 while the aqueous fraction had 5 μg/ml. On the inhibition of the guinea corn radicle growth, after 96 h, the controls had an average length of 67.81 ± 2.6 mm, whereas the seeds treated with 4 mg/ml of the crude extract had an average length of 35.83 ±1.75 mm, indicating 47.81% reduction in length. At the same concentration, the chloroform and the aqueous fractions showed 32.51 and 43.81% inhibitions. The plant material was observed to contain alkaloids, tannins, saponins and flavonoids, with no traces of anthracene derivatives. Conclusion: The results suggest the probable use of the plant in preparing recipes for tumor-related ailments.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Ayinde, B. A., & Agbakwuru, U. (2010). Cytotoxic and growth inhibitory effects of the methanol extract Struchium sparganophora Ktze (Asteraceae) leaves. Pharmacognosy Magazine, 6(24), 293–297. https://doi.org/10.4103/0973-1296.71795