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    Isolation and identification of phenolic compounds from Gynura divaricata leaves

    Chunpeng Wan2★, Yanying Yu3, Shouran Zhou4, Shuge Tian5, Shuwen Cao2,3★ Corresponding author

    1. 1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang-330047, Jiangxi, China.
    2. 2Department of Chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China.
    3. 3Jiangxi University of Traditional Chinese Medicine, Nanchang - 330 006, Jiangxi, China.
    4. 4Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, Xinjiang, China.

    CORRESPONDENCE

    Chunpeng Wan

    Department of Chemistry, Nanchang University, Nanchang - 330 031, Jiangxi, China.

    Received: 26-09-2010; Revised: 23-10-2010.

    Volume 7, Issue 26 · pp. 101–108 · PUBLISHED 9 May 2011 · DOI: 10.4103/0973-1296.80666

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Phenolic constituents were the principle bioactivity compounds exist in Gynura divaricata, little phenolic compounds were reported from the plant previously. Materials and Methods: 60% ethanol extract from the leaves of Gynura divaricata were isolated and purified by column chromatography of Silica gel, ODS and Sephadex LH-20, the structures of the isolated compounds were identified by UV, 1H-NMR, 13C-NMR and MS spectroscopic techniques. Additionally, a high-performance liquid chromatography-diode array detector-electrospray ionization-mass (HPLC-DAD-ESI-MS) analytical method was developed to identify some minor constituents in the n-butanol fraction of the ethanol extract of Gynura divaricata. Results: Six flavonols and one Dicaffeoylquinic acid were isolated from the leaves of Gynura divaricata, and these compounds were identified as follows: quercetin (1), kaempferol (2), kaempferol-3-O-β-d- glucopyranoside (3), quercetin-3-O-rutinoside (4), kaempferol-3,7-di-O-β-d-glucopyranoside (5), kaempferol-3-O-rutinoside-7-O-β-d-glucopyranoside (6), and 3,5-dicaffeoylquinic acid (7). A total of 13 compounds, including 9 flavonol glycosides and 4 phenolic acids, were tentatively identified by comparing their retention time (RT), UV, and MS spectrum values with those that had been identified and the published data. Conclusion: This was the first time to use the HPLC-DAD-ESI- MS method to identify the phytochemicals of the genera Gynura. Moreover, compounds (6) and (7) have been isolated for the first time from the genus Gynura.

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      Wan, C., Yu, Y., Zhou, S., Tian, S., & Cao, S. (2011). Isolation and identification of phenolic compounds from Gynura divaricata leaves. Pharmacognosy Magazine, 7(26), 101–108. https://doi.org/10.4103/0973-1296.80666