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    Article

    Isolation and characterization of a bactericidal withanolide from Physalis virginiana

    Kathleen A. Gibson1★, R. Neil Reese1, Fathi T. Halaweish2, Yulin Ren2★ Corresponding author

    1. 1Department of Biology and Microbiology, University, Brookings, SD, USA.
    2. 2Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD, USA.

    CORRESPONDENCE

    Kathleen A. Gibson

    Department of Biology and Microbiology, University, Brookings, SD, USA.

    Received: 06-05-2011; Revised: 15-06-2011.

    Volume 8, Issue 29 · pp. 22–28 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93307

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Physalis virginiana (Virginia Groundcherry) is a member of the family Solenaceae. Several species of the Physalis genus have been used traditionally by American Indians as medicinal treatments. Materials and Methods: This study investigated the antibacterial activity of chemicals extracted from P. virginiana through antibacterial disc and cytotoxicity assays. Isolation and purifi cation of an antimicrobial compound was achieved through fl ash chromatography and preparative HPLC. Finally, identifi cation of chemical structure was determined from 1H and 13C NMR and MS. Results: Disc assays showed that crude ethanol extracts were effective antibacterial agents against one gram-negative and seven gram-positive bacterial strains. Cytotoxicity assays indicated that it is less toxic than gentamicin controls. Isolation of the active component showed it to be a relatively polar compound. 1H and 13C NMR chemical shifts together with HRMS indicated a similar structure to withanolides previously identifi ed from Physalis angulata. HRMS analysis showed a molecular mass of 472.2857 which corresponds to a molecular formula C28H40O6. Conclusion: An antibacterial withanolide was isolated from P. virginiana using fl ash chromatography and HPLC separations. The chemical structure was determined by NMR and MS to be the withanolide physagulin V.

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      Gibson, K. A., Reese, R. N., Halaweish, F. T., & Ren, Y. (2012). Isolation and characterization of a bactericidal withanolide from Physalis virginiana. Pharmacognosy Magazine, 8(29), 22–28. https://doi.org/10.4103/0973-1296.93307