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    Article

    Application of deoxyribonucleic acid barcoding in Lauraceae plants

    Zhen Liu2,3★, Shi-Lin Chen4, Jing-Yuan Song4, Shou-Jun Zhang5, Ke-Li Chen3★ Corresponding author

    1. 1Department of Pharmacy, The 309th Hospital of Chinese People's Liberation Army, Beijing, China.
    2. 2Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.
    3. 3Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
    4. 4Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Republic of, China.

    CORRESPONDENCE

    Zhen Liu

    Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, China.

    Received: 12-04-2011; Revised: 07-05-2011.

    Volume 8, Issue 29 · pp. 4–11 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93301

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: This study aims to determine the candidate markers that can be used as DNA barcode in the Lauraceae family. Material and Methods: Polymerase chain reaction amplifi cation, sequencing effi ciency, differential intra- and interspecifi c divergences, DNA barcoding gap, and identifi cation effi ciency were used to evaluate the four different DNA sequences of psbA-trnH, matK, rbcL, and ITS2. We tested the discrimination ability of psbA-trnH in 68 plant samples belonging to 42 species from 11 distinct genera and found that the rate of successful identifi cation with the psbA-trnH was 82.4% at the species level. However, the correct identifi cation of matK and rbcL were only 30.9% and 25.0%, respectively, using BLAST1. The PCR amplifi cation effi ciency of the ITS2 region was poor; thus, ITS2 was not included in subsequent experiments. To verify the capacity of the identifi cation of psbA-trnH in more samples, 175 samples belonging to 117 species from the experimental data and from the GenBank database of the Lauraceae family were tested. Results: Using the BLAST1 method, the identifi cation effi ciency were 84.0% and 92.3% at the species and genus level, respectively. Conclusion: Therefore, psbA-trnH is confi rmed as a useful marker for differentiating closely related species within Lauraceae.

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      Liu, Z., Chen, S., Song, J., Zhang, S., & Chen, K. (2012). Application of deoxyribonucleic acid barcoding in Lauraceae plants. Pharmacognosy Magazine, 8(29), 4–11. https://doi.org/10.4103/0973-1296.93301