Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Biotransformation of furannoligularenone by transgenic crown galls of Panax quinquefolium

    Chunyan Yan2,3, Jue Wang4, Guoyan Duan3, Rongmin Yu3★★ Corresponding author

    1. 1Department of Natural Medicinal Chemistry, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
    2. 2Biotechnology Institute of Chinese Materia Medica, Jinan University, Guangzhou, China.
    3. 3Center of Forecasting and Analysis, Shenzhen Institute for Drug Control, Shenzhen, China.

    CORRESPONDENCE

    Rongmin Yu

    Center of Forecasting and Analysis, Shenzhen Institute for Drug Control, Shenzhen, China.

    tyrm@jnu.edu.cn

    Received: 12-08-2011; Revised: 14-09-2011.

    Volume 8, Issue 30 · pp. 124–128 · PUBLISHED 23 May 2012 · DOI: 10.4103/0973-1296.96555

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Transgenic plant suspension cultures could be used as an effective tool for the biotransformation of exogenous compounds. Objective: To investigate the biotransformation of furannoligularenone (1) by transgenic crown galls of Panax quinquefolium. Materials and Methods: Compound 1 was administered into the crown gall cultures and co- cultured for 6 days. The cultures were dried and extracted with methanol for HPLC analyses. The extract was separated on column chromatography, and biotransformation products’ structures were elucidated by the physicochemical properties and the data of NMR and MS. Moreover, three flasks were randomly chosen each day to establish time–course during the period for co- culturing. Results: Co-culturing compound 1 with crown galls yielded two compounds, 3-oxo- eremophila-1,7(11)-dien-12,8-olide (2) and 3-oxo-8-hydroxy-eremophila-1,7(11)-dien-12,8-olide (3), which were obtained by biotransformation using P. quinquefolium crown galls for the first time. Time–course investigation revealed that the mole conversion ratio reached the highest level of 45.5% and 33.9% on fourth and fifth day after substrate administration, respectively. Furthermore, a proposal biosynthesis pathway was given from compound 1 to compounds 2 and 3. Conclusion: This was the first example of compound 1 being successfully converted into compounds 2 and 3 by transgenic crown galls of P. quinquefolium.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Yan, C., Wang, J., Duan, G., & Yu, R. (2012). Biotransformation of furannoligularenone by transgenic crown galls of Panax quinquefolium. Pharmacognosy Magazine, 8(30), 124–128. https://doi.org/10.4103/0973-1296.96555