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    Article

    Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium

    Chun-Yan Guo2★, Jin Wang3★, Yong Hou3, Yong-Ming Zhao3, Li-Xia Shen3, Dan-Shen Zhang2,4★★ Corresponding author

    1. 1Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei, China.
    2. 2Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China.
    3. 3HeBei University of Science and Technology, Shijiazhuang, 050018, China.

    CORRESPONDENCE

    Chun-Yan Guo

    Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China.

    zhangds2011@126.com

    Received: 02-06-2012; Revised: 08-08-2012.

    Volume 9, Issue 35 · pp. 166–170 · PUBLISHED 11 June 2013 · DOI: 10.4103/0973-1296.113260

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. Materials and Methods: The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L9(34)) was then constructed to achieve the best extraction conditions. Results: Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. Conclusion: Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.

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      Guo, C., Wang, J., Hou, Y., Zhao, Y., Shen, L., & Zhang, D. (2013). Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium. Pharmacognosy Magazine, 9(35), 166–170. https://doi.org/10.4103/0973-1296.113260