Interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42)
Li Shuai1★, Zhi Chen2, Ruixi Gao1, Tianming Yang1★ Corresponding author
- 1College of Pharmacy, South-Central University for Nationalities, Minyuan Road 708, Hongshan District, Wuhan, Hubei, 430074, China.
- 2Center of Analysis, Guangdong Medical College, Dongguan, 523808, P. R, China.
CORRESPONDENCE
Li Shuai
College of Pharmacy, South-Central University for Nationalities, Minyuan Road 708, Hongshan District, Wuhan, Hubei, 430074, China.
Received: 20-06-2012; Revised: 03-10-2012.
Volume 9, Issue 35 · pp. 196–201 · PUBLISHED 11 June 2013 · DOI: 10.4103/0973-1296.113262
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ABSTRACT
Background: Lobed kudzuvine root and rhizoma chuanxiong are effective drugs in traditional Chinese medicine. Objective: Extracts of the two medicines were investigated for their in vitro of beta-amyloid (Aβ1-42)-aggregation-and acetylcholinesterase (AChE)-inhibitory activities. Materials and Methods: The interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42) were studied by Michaelis–Menten equations, Thioflavin T (ThT) fluorescence analysis and transmission electron microscope (TEM). Results: Inhibition of acetylcholinesterase showed that 1-butanol fraction of the two medicines were noncompetitive inhibition, apparent inhibition constants were 9.947 and 7.1523. ThT fluorescence analysis and TEM results indicated that inhibition of the water fraction and 1-butanol fraction (both lobed kudzuvine root and rhizoma chuanxiong) was better. Conclusion: The result supported further research on chemical constituents and pharmacological mechanisms.
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Shuai, L., Chen, Z., Gao, R., & Yang, T. (2013). Interaction of lobed kudzuvine root, rhizoma chuanxiong with both acetylcholinesterase and beta-amyloid (Aβ1-42). Pharmacognosy Magazine, 9(35), 196–201. https://doi.org/10.4103/0973-1296.113262