Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy ResearchNOW IN
    Article

    Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia

    Edna D. Molina-Romo1, Adriana Garibay-Escobar1, Olivia Valenzuela-Antelo1, Eduardo Ruiz-Bustos1, Javier Hernández Martínez2, Carlos Vélazquez1, Luisa A. Rascón-Valenzuela1, Ramón Enrique Robles-Zepeda1★★ Corresponding author

    1. 1Department of Chemical- Biological Sciences, University of Sonora, Hermosillo, Sonora, CALIFORNIA.
    2. 2Unit of Support Services in Analitycal Resolution, Veracruzana University, Xico, Veracruz, MEXICO.

    CORRESPONDENCE

    Ramón Enrique Robles-Zepeda

    Department of Chemical- Biological Sciences, University of Sonora, Hermosillo, Sonora, CALIFORNIA.

    rrobles@guayacan.uson.mx

    Volume 10, Issue 1 · pp. 55–59 · PUBLISHED · DOI: 10.4103/pr.pr_38_17

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background: Ziziphus obtusifolia is a spiny shrub found in Northwest Mexico desert, with traditional medicinal use to treat several diseases including cancer. Objective: The aims of the present study were to evaluate the antiproliferative and apoptotic activities of the aerial parts of this plant. Materials and Methods:The methanol extract and its fractions were prepared using several solvents. The antiproliferative activity was evaluated by the (3‑[4,5‑dimethylthiazol‑2‑yl]‑2,5‑diphenyltetrazolium) (MTT) assay on HeLa, A549, RAW 264.7, M12.C3.F6, and L‑929 cell lines, and the apoptotic activity using Annexin V and (5,50,6,60‑tetra‑chloro‑1,10,3,30‑tetra‑ethylbenzimidazol‑carbocyanine iodide) staining. The most active fraction was further separated by column chromatography. Results:The most active fraction was hexane with an IC50 of 90.4 µg/mL against RAW 264.7, 94 µg/mL against M12. C3.F6, 165.5 µg/mL against HeLa and 187.7 µg/mL against A549 cell lines. In apoptotic activity assays the methanol extract and its n‑hexane fraction were found to induce mitochondrial depolarization in HeLa cells (83 and 87% respectively), and both induced the externalization of the phosphatidylserine increasing the percentage of cells in early apoptosis from 1.4% in untreated control cells, to 1.9% and 3.5% for methanol extract and n‑hexane fraction‑treated cells, respectively, statistically different for the total percentage of apoptotic cells (P < 0.05). Conclusions:These results show thatZ. obtusifoliahas antiproliferative a

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Molina-Romo, E. D., Garibay-Escobar, A., Valenzuela-Antelo, O., Ruiz-Bustos, E., Martínez, J. H., Vélazquez, C., Rascón-Valenzuela, L. A., & Robles-Zepeda, R. E. (). Antiproliferative and Apoptotic Activities of the Medicinal Plant Ziziphus obtusifolia. Pharmacognosy Research, 10(1), 55–59. https://doi.org/10.4103/pr.pr_38_17