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    The Protective Effect of Different Extracts of Three Artemisia Species against H2O2‑Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells

    Leila Hosseinzadeh1, Alireza Malekshahi2, Farahnaz Ahmadi3, Seyed Ahmad Emami4, Marziyeh Hajialyani2, Mahdi Mojarrab2★★ Corresponding author

    1. 1Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, IRAN.
    2. 2School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, IRAN.
    3. 3Departments of Pharmacology and Toxicology and, IRAN.
    4. 4Pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IRAN.

    CORRESPONDENCE

    Mahdi Mojarrab

    School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, IRAN.

    mmojarrab@kums.ac.ir

    Volume 10, Issue 1 · pp. 64–71 · PUBLISHED · DOI: 10.4103/pr.pr_98_17

    View on Pharmacogn. Res. original site ↗

    ABSTRACT

    Background:Oxidative stress causes cell damage and is involved in many neurological diseases. The antioxidant properties of plant materials for the maintenance of health and protecting against different diseases stimulated scientist to investigate different herbs. Different Artemisia species have exhibited antioxidant activity. This study aims to investigate whether different Artemisia species could protect the PC12 cells against oxidative stress mediated by H2O2. Methods:For this purpose, different extracts of three Artemisia species (Artemisia aucheri, Artemisia turanica, and Artemisia turcomanica) were prepared using petroleum ether, dichloromethane, ethyl acetate, ethanol, and Water: Ethanol mixture (1:1 volume ratio). The protective effect of the prepared extracts against H2O2‑induced cytotoxicity and reactive oxygen species production were compared. The effect of treatment of PC12 cells with different extracts on total glutathione (GSH) level, caspase‑3 activity, and mitochondrial membrane potential were also compared. Results: The A. aucheri extracts could not rescue the PC12 cells from oxidative stress consequences. The A. turanica and A. turcomanica extracts were found potent in suppressing the toxicity and apoptosis of PC12 cells mediated by H2O2 and significantly antagonized the H2O2‑induced GSH depletion. The hydroethanolic and ethyl acetate extracts of A. turanica and the petroleum ether and hydroethanolic extracts of A. turcomanica more efficiently suppressed cytotoxicity and loss of GSH caused by H2O2. Conclusion: This study shows the protective effects of Artemisia extracts on PC12 cell line and suggested that these species could be also considered as promising neuroprotective agents in treatment of different neurodegenerative diseases. Key words: Artemisia aucheri, Artemisia turanica, Artemisia

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      Hosseinzadeh, L., Malekshahi, A., Ahmadi, F., Emami, S. A., Hajialyani, M., & Mojarrab, M. (). The Protective Effect of Different Extracts of Three Artemisia Species against H2O2‑Induced Oxidative Stress and Apoptosis in PC12 Neuronal Cells. Pharmacognosy Research, 10(1), 64–71. https://doi.org/10.4103/pr.pr_98_17